Paenibacillus dendrobii sp. nov., an indole-3-acetic acid-producing endophytic bacterium isolated from Dendrobium nobile. Issue 4 (11th April 2023)

• Record Type: Journal Article
• Authors: Hu, Yadong; Li, Hongjie; Chen, Yaoyi; Zhang, Qiling; Zheng, Shigang; Rao, Dan; Chun, Ze; Zhao, Ruoxi
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 4(2023)
• Publication Date: 2023-04-11
• Subjects: Dendrobium nobile -- endophyte -- novel species -- Paenibacillus dendrobii sp. nov.; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005824
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel rod-shaped, Gram-stain-positive, spore-forming and motile by peritrichous flagella strain, designated HJL G12 T, was isolated from the root of Chinese herb Dendrobium nobile . Strain HJL G12 T grew optimally at pH 7.0, 30 °C and in the presence of 1.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene and genomic sequences showed that HJL G12 T clustered with Paenibacillus chibensis NBRC 15958 T and Paenibacillus dokdonensis YH-JAE5 T with 98.3 and 98.2 % sequence similarity. The DNA–DNA hybridization values between strain HJL G12 T and the two reference strains were 23.6 % and 24.9 %, respectively. Menaquinone-7 was the only respiratory quinone and meso -diaminopimelic acid was present in the cell-wall peptidoglycan. Antesio-C15 : 0 and iso-C16 : 0 were detected to be the major cellular fatty acids. The cellular polar lipid profile contained diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phospatidylglycerol and three unidentified aminophospholipids. Based on these results, strain HJL G12 T is considered to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus dendrobii sp. nov. is proposed, with HJL G12 T (=NBRC 115617 T =CGMCC 1.18520 T ) as the type strain.