Rathayibacter rubneri sp. nov. isolated from Allium cepa var. Rijnsburger, an onion landrace. Issue 4 (11th April 2023)

• Record Type: Journal Article
• Authors: Stoll, Dominic A.; Grimmler, Christina; Hetzer, Birgit; Kulling, Sabine E.; Huch, Melanie
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 4(2023)
• Publication Date: 2023-04-11
• Subjects: Microbacteriaceae -- onion landrace -- Rathayibacter; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005811
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : The novel, aerobic, Gram-stain-positive, rod-shaped bacterial strain, ZW T2_19 T, was isolated from an onion sample ( Allium cepa var. Rijnsburger). Analyses of the 16S rRNA gene sequence revealed that ZW T2_19 T represented a member of the genus Rathayibacter but may represent a novel species of this genus. Analyses of the whole draft genome sequences, i.e. digital DNA–DNA hybridisation (dDDH) and average nucleotide identity (ANI) of ZW T2_19 T and all type strains of species of the genus Rathayibacter confirmed that ZW T2_19 T represents a novel species of the genus Rathayibacter . The genome size of ZW T2_19 T is 4.01 Mbp and the DNA G+C content is 71.8 mol%. Glucose, mannose, rhamnose and ribose were detected as whole-cell sugars of ZW T2_19 T . The major respiratory quinone of ZW T2_19 T is menaquinone MK-10, at 78.9 %. The detected peptidoglycan type in ZW T2_19 T is a variant of type B2γ with {Gly} [l -diaminobutyric acid (l -DAB)/l -homoserine (l -Hse)] d -Glu-l -DAB. Polar lipids in ZW T2_19 T consisted of one diphosphatidylglycerol, one phosphatidylglycerol, seven glycolipids, one phospholipid and one lipid. The fatty acid profile of ZW T2_19 T predominantly consisted of anteiso-C15 : 0 (53 %), iso-C16 : 0 (21 %) and anteiso-C17 : 0 (18 %). In addition, API 20NE, API 50CH, API Coryne, API ZYM, antibiotic susceptibility, haemolysis and growth at different temperatures and with different supplements was investigated. On the basis of the results obtained using this polyphasic approach, including molecular, phenotypic and biochemical analyses, we propose the novel species Rathayibacter rubneri with the type and only strain ZW T2_19 T (= DSM 114294 T = LMG 32700 T ).