Aqueous Rosa damascena extract: Antibacterial activity and its role of adhesion to human epithelial cells in vitro. (14th March 2023)

• Record Type: Journal Article
• Authors: Alsafi, Alaa; AlKaabi, Siham Jasim
• Is Part Of: Cell biochemistry and function. Volume 41:Number 3(2023)
• Page Start: 365
• Page End: 374
• Publication Date: 2023-03-14
• Subjects: antimicrobial activity -- epithelial cells -- Rosa damascena -- synergistic act; Cytochemistry -- Periodicals; Cell metabolism -- Periodicals; Biochemistry -- Periodicals; Cytology -- Periodicals; 572
• DOI: 10.1002/cbf.3788
• Languages: English
• ISSNs: 0263-6484

Abstract

Abstract: The current study aimed to investigate the inhibitory activity of aqueous extracts of different plant parts of Rosa damascena, represented by the whole rose, petals, and calyces, against clinical isolates of Staphylococcus spp ., Escherichia coli, and Klebsiella pneumoniae, and the inhibition of the bacterial cell. The isolates were obtained from the advanced microbiology laboratory for postgraduate studies in the Department of Biology, Faculty of Education for Girls. They were isolated from urinary tract infections, which were subsequently subjected to diagnosis by the Vitek‐2 compact system to confirm the type of bacteria as well as their sensitivity to antibiotics. The results obtained included Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus lentus, Staphylococcus saprophyticus, E. coli, and K. pneumoniae . A test was conducted to investigate the microbiological inhibitory activity of aqueous plant extracts of the whole rose, petals, and calyces using the well diffusion method and three concentrations of each aqueous extract (25, 50, and 100 mg/ml). The results showed the inhibitory ability of all concentrations of the different extracts toward Staphylococcus spp ., and E. coli and K. pneumoniae bacteria were not affected by the different concentrations of the plant extract. The concentration of (100 mg/ml) for the aqueous extract was the most efficient in inhibiting growth compared to the other concentrations. The synergistic effect of three antibiotics was examined (Amoxicillin‐clavulanate 10/20 μg, Piperacillin 100 μg, Trimethoprim‐sulfamethoxazole 23.75/1.25 μg) and for all concentrations of the aqueous plant extract was investigated in both E. coli and K. pneumoniae, as it found a synergistic action between some of the antibiotics and extracts towards inhibiting the growth of the two bacterial isolates Resistance to the plant extract alone. Bacterial isolates showed a significant decrease in the rate of adhesion to epithelial cells isolated from urine samples of healthy women in the presence of the aqueous extract of whole rose, petals, and calyces at their three concentrations compared with the control treatment. Highlights: This study aims to explore the inhibitory properties of aqueous extracts of Rosa damascena at various concentrations against Staphylococcus spp . bacterial isolates as well as E. coli and K. pneumonia . The study will investigate if there is any synergistic action between the Rosa damascena extracts and three types of antibiotics (Amoxicillin‐clavulanate, Piperacillin, and Trimethoprim‐sulfamethoxazole) on the two bacterial isolates E. coli and K. pneumonia . The study will observe the impact of wild Rosa damascena extracts on preventing bacterial cells from adhering to epithelial cells in the urinary tract.