Integrated biochemical and transcriptomic analysis reveals the effects of Burkholderia sp. SRB-1 on cadmium accumulating in Chrysopogon zizanioides L. under Cd stress. (1st July 2023)

• Record Type: Journal Article
• Authors: Liu, Huakang; Huang, Huayan; Xie, Yanluo; Liu, Yikai; Shangguan, Yuxian; Xu, Heng
• Is Part Of: Journal of environmental management. Volume 337(2023)
• Publication Date: 2023-07-01
• Subjects: Cadmium -- Chrysopogon zizanioides -- Plant growth promoting rhizobacteria -- Transcriptomic analysis -- Phytoremediation; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2023.117723
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: Application of plant growth-promoting rhizobacteria plays a vital role in enhancing phytoremediation efficiency. In this study, multiple approaches were employed to investigate the underlying mechanisms of Burkholderia sp. SRB-1 (SRB-1) on elevating Cd uptake and accumulation. Inoculation experiment indicated that SRB-1 could facilitate plant growth and Cd tolerance, as evidenced by the enhanced plant biomass and antioxidative enzymes activities. Cd content in plant shoots and roots increased about 36.56%–39.66% and 25.97%–130.47% assisted with SRB-1 when compared with control. Transcriptomics analysis revealed that SRB-1 upregulated expression of amiE, AAO1-2 and GA2-ox related to auxin and gibberellin biosynthesis in roots. Auxin and gibberellin, as hormone signals, regulated plant Cd tolerance and growth through activating hormone signal transduction pathways, which might also contribute to 67.94% increase of dry weight. The higher expression levels of ATP-binding cassette transporter subfamilies (ABCB, ABCC, ABCD and ABCG) in Chrysopogon zizanioides roots contributed to higher Cd uptake in Cd15 B (323.83 mg kg −1 ) than Cd15 (136.28 mg kg −1 ). Further, SRB-1 facilitated Cd migration from roots to shoots via upregulating the expression of Nramp, ZIP and HMA families. Our integrative analysis provided a molecular-scale perspective on Burkholderia sp. SRB-1 contributing to C. zizanioides performance. Graphical abstract: Image 1 Highlights: Burkholderia sp. SRB-1 could facilitate plant growth, Cd-tolerance and Cd uptake. SRB-1 obviously upregulated expression of amiE, AAO1-2 and GA2-ox in plant roots. Auxin and gibberellin, as signaling molecular, regulate accumulator performance. SRB-1 facilitated Cd migration via upregulating the expression of ABC families.