Improved cellular response on functionalized polypyrrole interfaces. Issue 9 (30th January 2019)

• Record Type: Journal Article
• Authors: Naghavi Alhosseini, Sanaz; Moztarzadeh, Fathollah; Karkhaneh, Akbar; Dodel, Masumeh; Khalili, Mahsa; Eslami Arshaghi, Tarlan; Elahirad, Elnaz; Mozafari, Masoud
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 9(2019:Sep.)
• Page Start: 15279
• Page End: 15287
• Publication Date: 2019-01-30
• Subjects: electroactive scaffold -- gelatin -- polypyrrole -- tissue engineering; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28173
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Neuroregeneration strategies involve multiple factors to stimulate nerve regeneration. Neural support with chemical and physical cues to optimize neural growth and replacing the lesion neuron and axons are crucial for designing neural scaffolds, which is a promising treatment approach. In this study, polypyrrole polymerization and its functionalization at the interface developed by glycine and gelatin for further optimization of cellular response. Nanofibrous scaffolds were fabricated by electrospinning of polyvinyl alcohol and chitosan solutions. The electrospun scaffolds were polymerized on the surface by pyrrole monomers to form an electroactive interface for further applications in neural tissue engineering. The polymerized polypyrrole showed a positive zeta potential value of 57.5 ± 5.46 mV. The in vitro and in vivo biocompatibility of the glycine and gelatin‐functionalized polypyrrole‐coated scaffolds were evaluated. No inflammatory cells were observed for the implanted scaffolds. Further, DAPI nucleus staining showed a superior cell attachment on the gelatin‐functionalized polypyrrole‐coated scaffolds. The topography and tuned positively charged polypyrrole interface with gelatin functionalization is expected to be particularly efficient physical and chemical simultaneous factors for promoting neural cell adhesion. Abstract : The electrospun PVA–chitosan fibers were coated by polypyrrole polymerization as an electroactive interface. Functionalization of polypyrrole interface to boost cell interactions was carried by glycine and gelatin. The effect of functionalized conductive interfaces was compared in vitro and in vivo.