Blocking the IFN‐gamma signal in the choroid plexus confers resistance to experimental autoimmune encephalomyelitis. Issue 4 (15th March 2023)

• Record Type: Journal Article
• Authors: Zheng, Yuyin; Hu, Lanxin; Yang, Yuwen; Zheng, Cheng; Tu, Wenzhan; Lin, Haiyan; Wang, Haotian; Jiang, Yiwei; Jiang, Songhe; Zheng, Wu
• Is Part Of: FASEB journal. Volume 37:Issue 4(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-03-15
• Subjects: choroid plexus -- CNS immune cell infiltration -- experimental autoimmune encephalomyelitis -- IFN‐γ -- IFN‐γR1; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.202201767R
• Languages: English
• ISSNs: 0892-6638

Abstract

Abstract: Multiple sclerosis (MS) is an autoimmune disease characterized by inflammatory infiltration and demyelination in the central nervous system (CNS). IFN‐gamma (IFN‐γ), a critically important immunomodulator, has been widely studied in MS pathology. The confusing and complex effects of IFN‐γ in MS patients and rodent models, however, cause us to look more closely at its exact role in MS. In this study, we identified the role of the IFN‐γ signaling in the choroid plexus (CP) in the experimental autoimmune encephalomyelitis (EAE) model. We found that the IFN‐γ signal was rapidly amplified when CNS immune cell infiltration occurred in the CP during the progressive stage. Furthermore, using two CP‐specific knockdown strategies, we demonstrated that blocking the IFN‐γ signal via knockdown of IFN‐γR1 in the CP could protect mice against EAE pathology, as evidenced by improvements in clinical scores and infiltration. Notably, knocking down IFN‐γR1 in the CP reduced the local expression of adhesion molecules and chemokines. This finding suggests that IFN‐γ signaling in the CP may participate in the pathological process of EAE by preventing pathological T helper (Th) 17 + cells from infiltrating into the CNS. Finally, we showed that the unbalanced state of IFN‐γ signaling between peripheral lymphocytes and the choroid plexus may determine whether IFN‐γ has a protective or aggravating effect on EAE pathology. Above all, we discovered that IFN‐γR1‐mediated IFN‐γ signaling in the CP was a vital pathway in the pathological process of EAE. Abstract : We identified the role of the IFN‐γ signaling in the choroid plexus (CP) in the experimental autoimmune encephalomyelitis (EAE) model. We found that the IFN‐γ signal was rapidly amplified when CNS immune cell infiltration occurred in the CP during the progressive stage. With CP‐specific knockdown strategy, we demonstrated that blocking the IFN‐γR1 signal in the CP could protect mice against EAE pathology, as evidenced by improvement in clinical scores and infiltration of T cells.