LncRNA‐ATB promotes TGF‐β‐induced glioma cells invasion through NF‐κB and P38/MAPK pathway. Issue 12 (29th May 2019)

• Record Type: Journal Article
• Authors: Tang, Feng; Wang, Hongliang; Chen, Erfeng; Bian, Erbao; Xu, Yadi; Ji, Xinghu; Yang, Zhihao; Hua, Xiangyang; Zhang, Yile; Zhao, Bing
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 12(2019:Dec.)
• Page Start: 23302
• Page End: 23314
• Publication Date: 2019-05-29
• Subjects: glioma -- invasion -- lncRNA‐ATB -- NF‐κB -- P38/MAPK -- TGF‐β; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28898
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Glioma constitutes the most aggressive primary intracranial malignancy in adults. We previously showed that long noncoding RNA activated by TGF‐β (lncRNA‐ATB) promoted the glioma cells invasion. However, whether lncRNA‐ATB is involved in TGF‐β‐mediated invasion of glioma cells remains unknown. In this study, quantitative real‐time polymerase chain reaction and western blot analysis were used for detecting the mRNA and protein expression of related genes, respectively. Transwell assay was performed to assess the impact of lncRNA‐ATB on TGF‐β‐induced glioma cells migration and invasion. Immunofluorescence staining was utilized to characterize related protein distribution. Results showed that TGF‐β upregulated lncRNA‐ATB expression in glioma LN‐18 and U251 cells. Overexpression of lncRNA‐ATB activated nuclear factor‐κB (NF‐κB) pathway and promoted P65 translocation into the nucleus, thus facilitated glioma cells invasion stimulated by TGF‐β. Similarly, lncRNA‐ATB markedly enhanced TGF‐β‐mediated invasion of glioma cells through activation P38 mitogen‐activated protein kinase (P38/MAPK) pathway. Moreover, both the NF‐κB selected inhibitor pyrrolidinedithiocarbamate ammonium and P38/MAPK specific inhibitor SB203580 partly reversed lncRNA‐ATB induced glioma cells invasion mediated by TGF‐β. Collectively, this study revealed that lncRNA‐ATB promotes TGF‐β‐induced glioma cell invasion through NF‐κB and P38/MAPK pathway and established a detailed framework for understanding the way how lncRNA‐ATB performs its function in TGF‐β‐mediated glioma invasion. Abstract : Long noncoding RNA activated by TGF‐β (lncRNA‐ATB) activated by TGF‐β promoted glioma cells migration and invasion induced by TGF‐β via activating nuclear factor‐κB (NF‐κB) and P38 mitogen‐activated protein kinase (P38/MAPK) pathway. Both pyrrolidinedithiocarbamate ammonium (PDTC) and SB203580 partly reversed lncRNA‐ATB‐mediated glioma invasion induced by TGF‐β through inhibiting NF‐κB and P38/MAPK pathway respectively.