Evidence of combined effect of amino acid substitutions within G‐H and B‐C loops of VP1 conferring serological heterogeneity in foot‐and‐mouth disease virus serotype A. Issue 2 (5th July 2020) - Islam, M. Rafiul
Abstract: Foot‐and‐mouth disease virus (FMDV) serotype A exhibits a higher degree of genetic and antigenic diversity resulting in frequent vaccine failure due to serological mismatch between the vaccine and heterologous strains. Currently, knowledge on the molecular basis of antigenic relationships among the FMDVs is limited; nevertheless, intratype antigenic variation due to mutation(s) is widely considered as the main hurdle to appropriate FMD vaccine development. Here, we studied genetic and antigenic variations of four FMDV serotype A isolates, BAN/GA/Sa‐197/2013 (BAN‐197), BAN/CH/Sa‐304/2016 (BAN‐304), BAN/DH/Sa‐307/2016 (BAN‐307) and BAN/DH/Sa‐310/2017 (BAN‐310) circulating in Bangladesh during 2013–2017. Initially, antigenic relationships (r1 ‐values) of the field isolates were evaluated by the two‐dimensional microneutralization test (2D‐MNT) using the hyperimmune antisera raised in cattle against the vaccine strain, BAN‐304. Interesingly, the results showed protective serological cross‐reactivity (r1 ‐values > 0.4) between the vaccine strain and the field isolates, BAN‐307 and BAN‐310, except BAN‐197 that substantially mismatched (r1 = 0.129 ± 0.043) with the BAN‐304. Although VP1‐based phylogeny grouped all the isolates within the same sublineage C (a subgroup of VP3 Δ59 variant) under the lineage A/ASIA/G‐VII, strikingly, computational analyses of the viral capsid proteins demonstrated significant deviation at the VP1 G‐H loop of BAN‐197 from the vaccine strain, while VP(2–4) of both isolates were structurally conserved. To bridge the gap of how the distortion of the G‐H loop and consequent antigenic hetergeneity occurred in BAN‐197, we performed in silico combinatorial substitutions of the VP1 mutant amino acids (aa) of BAN‐197 with the respective residues in BAN‐304. Remarkably, our analyses revealed that two substitutions of distantly located aa at B‐C (T48I:threonine → isoleucine) and G‐H (A143V:alanine → valine) loops, in combination, distorted the VP1 G‐H loop. Overall, this work contributes to understanding the molecular basis of antigenic relationships operating in serotype A FMDVs and the selection of suitable vaccine strain(s) for effective prophylaxis of FMD based on VP1‐based analyses.