A variant in CYP2R1 predicts circulating vitamin D levels after supplementation with high‐dose of vitamin D in healthy adolescent girls. Issue 8 (9th January 2019)

• Record Type: Journal Article
• Authors: Khayyatzadeh, Sayyed Saeid; Mehramiz, Mehrane; Esmaeily, Habibollah; Mirmousavi, Seyed Jamal; Khajavi, Leila; Salehkhani, Fatemeh Nejati; Hanachi, Parichehr; Bahrami‐Taghanaki, Hamidreza; Eslami, Saeed; Vatanparast, Hasan; Ferns, Gordon A.; Avan, Amir; Ghayour‐Mobarhan, Majid
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 8(2019:Aug.)
• Page Start: 13977
• Page End: 13983
• Publication Date: 2019-01-09
• Subjects: CYP2R1 -- rs10741657 -- supplementation -- vitamin D; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28083
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Aim: The determinants of serum vitamin D seems to be the environmental factors (dietary and supplementary intake and exposure to ultraviolet light) and genetic factors. We aimed to study the relationship between a vitamin D ‐associated genetic polymorphism and serum 25(OH)D concentrations in healthy adolescent girls in Iran, and its effects on a high‐dose supplement of vitamin D. Material and method: A total of 616 healthy adolescent girls with mean age 15 received 50, 000 IU of vitamin D3 weekly over 9 weeks. Serum vitamin D levels and other metabolic factors were measured at baseline and after the intervention. The genotyping of the CYP2R1 variant (rs10741657) was performed by TaqMan genotyping assays. Results: Regardless of the genetic background, at baseline, 87% of adolescent girls were vitamin D deficient (serum 25(OH)D level < 50 nmol/l). High‐dose supplementation with VitD reduced the proportion of girls who were deficient substantially to about 24%. The genetic analysis revealed that although at baseline there was not a gene‐vitamin D association ( p trend = 0.1), the response to supplementation appeared to be modulated by this variant ( p trend < 0.001). However, other anthropometric and biochemical measures were not affected by this intervention, over this short period. Serum 25(OH)D was increased in all participants although the carriers of the minor A allele seemed to be better responders so that the percentages of the change serum vitamin D in the holder of AA and AG genotypes were 539.4 ± 443.1 and 443.7 ± 384.6, respectively, compared with those with common GG genotype (363.3 ± 354.0). Our regression analysis revealed that the probability of an increase in serum 25(OH)D in a participant with AA genotype was 2.5‐fold greater than those with a GG genotype (OR = 2.5 (1.4–4.4); p value = 0.002). Conclusion: Based on our findings, it appears that the rs10741657 variant of the CYP2R1 gene modulates the response to high‐dose of vitamin D supplementation.