LncRNA CRNDE regulates the proliferation and migration of vascular smooth muscle cells. Issue 9 (10th February 2019)

• Record Type: Journal Article
• Authors: Zhou, Yu; He, Xuyu; Liu, Ruiming; Qin, Yuansen; Wang, Shenming; Yao, Xi; Li, Chunying; Hu, Zuojun
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 9(2019:Sep.)
• Page Start: 16205
• Page End: 16214
• Publication Date: 2019-02-10
• Subjects: LncRNA CRNDE -- microarray -- migration -- proliferation -- vascular smooth muscle cell; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28284
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Restenosis after angioplasty or stent is a major clinical problem. While long noncoding RNAs (lncRNAs) are implicated in a variety of diseases, their role in restenosis is not well understood. This study aims to investigate how dysregulated lncRNAs and messenger RNAs (mRNAs) contribute to restenosis. By microarray analysis, we identified 202 lncRNAs and 625 mRNAs (fold change > 2.0, p < 0.05) differentially expressed between the balloon‐injured carotid artery and uninjured carotid artery in the rats. Among differentially expressed lncRNAs, LncRNA CRNDE had the highest fold change and the change was validated by reverse transcription polymerase chain reaction. We found that LncRNA CRNDE was significantly upregulated in injured rat carotid artery and vascular smooth muscle cells (VSMCs) stimulated by platelet‐derived growth factor‐BB (PDGF‐BB). Knockdown of LncRNA CRNDE by small interference RNA significantly inhibited PDGF‐BB stimulated proliferation and migration of VSMCs. Moreover, knockdown of LncRNA CRNDE attenuated PDGF‐BB‐induced phenotypic change of VSMCs. Taken together, our study reveals a novel mechanoresponsive LncRNA CRNDE which may be a therapeutic target for restenosis. Abstract : Our study is the first to explore long noncoding RNAs (lncRNA) expression profile in rat carotid artery balloon injury model by microarray. In particular, we revealed the role of LncRNA CRNDE in regulating the proliferation and migration as well as a phenotypic change of vascular smooth muscle cells (VSMCs). Our findings will help to identify the new diagnostic and therapeutic targets for vascular restenosis.