Members of the ribosomal protein S6 (RPS6) family act as pro‐viral factor for tomato spotted wilt orthotospovirus infectivity in Nicotiana benthamiana. (16th December 2021)

• Record Type: Journal Article
• Authors: Helderman, Tieme A.; Deurhof, Laurens; Bertran, André; Richard, Manon M. S.; Kormelink, Richard; Prins, Marcel; Joosten, Matthieu H. A. J.; van den Burg, Harrold A.
• Is Part Of: Molecular plant pathology. Volume 23:Number 3(2022)
• Page Start: 431
• Page End: 446
• Publication Date: 2021-12-16
• Subjects: disease susceptibility -- forward genetics -- genetic screen -- tomato spotted wilt virus -- Tospoviridae -- viral susceptibility factors -- virus‐induced gene silencing; Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.13169
• Languages: English
• ISSNs: 1464-6722

Abstract

Abstract: To identify host factors for tomato spotted wilt orthotospovirus (TSWV), a virus‐induced gene silencing (VIGS) screen using tobacco rattle virus (TRV) was performed on Nicotiana benthamiana for TSWV susceptibility. To rule out any negative effect on the plants' performance due to a double viral infection, the method was optimized to allow screening of hundreds of clones in a standardized fashion. To normalize the results obtained in and between experiments, a set of controls was developed to evaluate in a consist manner both VIGS efficacy and the level of TSWV resistance. Using this method, 4532 random clones of an N. benthamiana cDNA library were tested, resulting in five TRV clones that provided nearly complete resistance against TSWV. Here we report on one of these clones, of which the insert targets a small gene family coding for the ribosomal protein S6 (RPS6) that is part of the 40S ribosomal subunit. This RPS6 family is represented by three gene clades in the genome of Solanaceae family members, which were jointly important for TSWV susceptibility. Interestingly, RPS6 is a known host factor implicated in the replication of different plant RNA viruses, including the negative‐stranded TSWV and the positive‐stranded potato virus X. Abstract : An optimized tobacco rattle virus‐mediated virus‐induced gene silencing screen in Nicotiana benthamiana is used to find pro‐viral factors for Tomato spotted wilt orthotospovirus .