LncRNA GAS5 promotes M1 macrophage polarization via miR‐455‐5p/SOCS3 pathway in childhood pneumonia. Issue 8 (24th December 2018)

• Record Type: Journal Article
• Authors: Chi, Xiaowen; Ding, Beichen; Zhang, Lijuan; Zhang, Jiawen; Wang, Jianmei; Zhang, Wei
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 8(2019:Aug.)
• Page Start: 13242
• Page End: 13251
• Publication Date: 2018-12-24
• Subjects: JAK2/STAT3 -- lncRNA GAS5 -- macrophage polarization -- miR‐455‐5p -- pneumonia -- SOCS3; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27996
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Objectives: We herein aimed to explore whether growth arrest‐specific 5 (GAS5) promotes M1 macrophage polarization in childhood pneumonia and to investigate the underlying mechanism. Methods: Relative GAS5 and miR‐455‐5p expression and suppressor of cytokine signaling 3 (SOCS3) messenger RNA level were examined using quantitative reverse transcription polymerase chain reaction. Protein expression of SOCS3 and the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway‐related proteins was detected using western blot analysis. Luciferase activity assay was performed to test whether miR‐455‐5p could bind to GAS5 or SOCS3. The macrophage phenotype was determined using flow cytometry analysis and enzyme‐linked immunosorbent assay. Results: The macrophage polarization toward the M2 phenotype was observed in peripheral blood from pneumonia children. Furthermore, GAS5 and SOCS3 expression were upregulated but miR‐455‐5p downregulated in human monocyte‐derived macrophages from pneumonia children compared with the control group. Furthermore, GAS5 acted as a sponge for miR‐455‐5p to facilitate SOCS3 expression. Moreover, miR‐455‐5p mimic and SOCS3 knockdown significantly reversed the GAS5 overexpression‐mediated suppression of the JAK2/STAT3 signaling and promotion of M1 polarization. Conclusion: GAS5 promotes M1 macrophage polarization by acting as a competing endogenous RNA of miR‐455‐5p to facilitate SOCS3 expression in childhood pneumonia. Abstract : Growth arrest‐specific 5 (GAS5) promotes M1 macrophage polarization by acting as a competing endogenous RNA of miR‐455‐5p to facilitate suppressor of cytokine signaling 3 (SOCS3) expression in childhood pneumonia.