Cooling therapy upregulates protein C activation in heat stressed rats: An experimental study. Issue 8 (11th January 2019)

• Record Type: Journal Article
• Authors: Gao, Tie‐Ying; Yang, Wen‐Chao; Zhou, Fei‐Hu; Song, Qing
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 8(2019:Aug.)
• Page Start: 14181
• Page End: 14186
• Publication Date: 2019-01-11
• Subjects: activated protein C -- heat stress -- protein C -- soluble thrombomodulin; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28114
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Protein C (PC) pathway homeostasis is implicated in heat stress (HS). This study determines whether cooling could improve the PC pathway in HS. Fifty‐six anesthetized rats were warmed to achieve HS (rectal temperature [ Tr ] 42°C). These rats were divided into seven groups: (a) control group:sacrifice immediately 15 min after HS; (b) HS+I:sacrifice immediately after 15 min ice‐water treatment or (c) 3 hr after HS; (d) HS+C:sacrifice immediately after 15‐min cold‐water treatment or (e) 3 hr after HS; (f) HS: sacrifice immediately 15 min after HS or (g) 3 hr after HS. Plasma PC, activated protein C (APC), and soluble thrombomodulin (sTM) levels were tested at both time points. After cooling, Tr in the HS+I and HS+C groups significantly decreased, when compared with the HS group, and Tr was significantly lower in the HS+I group than in the HS+C group ( p < 0.05). Furthermore, sTM levels were highest in the HS group among the groups at both time points. Plasma PC and APC levels increased after HS. In the HS+I and HS+C groups, plasma APC levels and the APC/PC ratio significantly increased at both time points. The proportions were significantly higher in the HS+I group than in the HS+C group, and there was no significant increase in APC/PC ratio in the HS group. Cooling exerts an anticoagulant effect following HS by increasing APC levels. Ice‐water blanket therapy is more effective than cold‐water blanket therapy in increasing APC levels. Abstract : Protein C (PC) pathway homeostasis is implicated in heat stress (HS). This study determines whether cooling could improve the PC pathway in HS. Cooling exerts an anticoagulant effect following HS by increasing activated protein C (APC) levels. Ice‐water blanket therapy is more effective than cold‐water blanket therapy in increasing APC levels.