Novel microbiome signatures for non‐invasive diagnosis of adenoma recurrence after colonoscopic polypectomy. Issue 7 (27th February 2022)

• Record Type: Journal Article
• Authors: Liang, Jessie Qiaoyi; Zeng, Yao; Kwok, Grace; Cheung, Chun Pan; Suen, Bing Yee; Ching, Jessica Y. L.; To, Ka Fai; Yu, Jun; Chan, Francis K. L.; Ng, Siew Chien
• Is Part Of: Alimentary pharmacology & therapeutics. Volume 55:Issue 7(2022)
• Page Start: 847
• Page End: 855
• Publication Date: 2022-02-27
• Subjects: Digestive organs -- Diseases -- Treatment -- Periodicals; Digestive organs -- Effect of drugs on -- Periodicals; Gastrointestinal system -- Diseases -- Treatment -- Periodicals; Gastrointestinal system -- Effect of drugs on -- Periodicals; 615.73
• DOI: 10.1111/apt.16799
• Languages: English
• ISSNs: 0269-2813

Abstract

Summary: Background: We previously reported a panel of novel faecal microbiome gene markers for diagnosis of colorectal adenoma and cancer. Aim: To evaluate whether these markers are useful in detecting adenoma recurrence after polypectomy. Methods: Subjects were enrolled in a polyp surveillance study from 2009 to 2019. Stool samples were collected before bowel preparation of index colonoscopy (baseline) and surveillance colonoscopy (follow‐up). Fusobacterium nucleatum ( Fn ), Lachnoclostridium marker ( m3 ), Clostridium hathewayi ( Ch ) and Bacteroides clarus were quantified in baseline and follow‐up samples by quantitative polymerase chain reaction (qPCR) to correlate with adenoma recurrence. Recurrence was defined as new adenomas detected >6 months after polypectomy. Faecal immunochemical test (FIT) was performed for comparison. Results: A total of 161 baseline and 104 follow‐up samples were analysed. Among patients with adenoma recurrence, Fn and m3 increased (both P < 0.05) while Ch were unchanged in follow‐up versus baseline samples. Among patients without recurrence, Fn and m3 were unchanged while Ch decreased ( P < 0.05) in follow‐up versus baseline samples. Logistic regression that included changes of m3, Fn and Ch at follow‐up compared with baseline achieved an area under receiver operating characteristic curve (AUROC) of 0.95 (95%CI: 0.84‐0.99) with 90.0% sensitivity and 87.0% specificity for detecting recurrent adenoma. Combination of m3, Fn and Ch at follow‐up sample achieved AUROC of 0.74 (95%CI: 0.65‐0.82) with 81.3% sensitivity and 55.4% specificity for detecting recurrent adenoma. FIT showed limited sensitivity (8.3%) in detecting recurrent adenomas. Conclusion: Our combinations of faecal microbiome gene markers can be potentially useful non‐invasive tools for detecting adenoma recurrence. Abstract : Faecal microbiome gene markers can be potentially useful non‐invasive tools for detecting adenoma recurrence.