Phosphoinositide‐dependent kinase 1–associated glycolysis is regulated by miR‐409‐3p in clear cell renal cell carcinoma. Issue 1 (14th September 2018)

• Record Type: Journal Article
• Authors: Wang, Yongjun; He, Yanfa; Bai, Hongzhong; Dang, Yi; Gao, Jiangyan; Lv, Pei
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 1(2019)
• Page Start: 126
• Page End: 134
• Publication Date: 2018-09-14
• Subjects: clear cell renal cell carcinoma -- glycolysis -- miR‐409‐3p -- phosphoinositide‐dependent kinase 1; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.27152
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Clear cell renal cell carcinoma (ccRCC) is the most popular kidney cancer in adults. Metabolic shift toward aerobic glycolysis is a fundamental factor for ccRCC therapy. MicroRNAs (miRNAs) are thought to be important regulators in ccRCC development and progression. Phosphoinositide‐dependent kinase 1 (PDK1) is required for metabolic activation; however, the role of PDK1‐induced glycolytic metabolism regulated by miRNAs is unclear in ccRCC. So, the purpose of the current study is to elucidate the underlying mechanism in ccRCC cell metabolism mediated by PDK1. Our results revealed that miR‐409‐3p inhibited glycolysis by regulating PDK1 expression in ccRCC cells. We also found that miR‐409‐3p was regulated by hypoxia. Our results indicated that PDK1 facilitated ccRCC cell glycolysis, regulated by miR‐409‐3p in hypoxia. Abstract : MiR‐409‐3p is a target gene of phosphoinositide‐dependent kinase 1 in clear cell renal cell carcinoma (ccRCC) cells and tissues, which is downregulated by hypoxia/hypoxia‐inducible factor 1‐α. MiR‐409‐3p decreases ccRCC cell survival ability and glycolysis.