A high‐temperature water vapor equilibration method to determine non‐exchangeable hydrogen isotope ratios of sugar, starch and cellulose. (30th September 2021) - Schuler, Philipp
Abstract: The analysis of the non‐exchangeable hydrogen isotope ratio (δ 2 Hne ) in carbohydrates is mostly limited to the structural component cellulose, while simple high‐throughput methods for δ 2 Hne values of non‐structural carbohydrates (NSC) such as sugar and starch do not yet exist. Here, we tested if the hot vapor equilibration method originally developed for cellulose is applicable for NSC, verified by comparison with the traditional nitration method. We set up a detailed analytical protocol and applied the method to plant extracts of leaves from species with different photosynthetic pathways (i.e., C3, C4 and CAM). δ 2 Hne of commercial sugars and starch from different classes and sources, ranging from −157.8 to +6.4‰, were reproducibly analysed with precision between 0.2‰ and 7.7‰. Mean δ 2 Hne values of sugar are lowest in C3 (−92.0‰), intermediate in C4 (−32.5‰) and highest in CAM plants (6.0‰), with NSC being 2 H‐depleted compared to cellulose and sugar being generally more 2 H‐enriched than starch. Our results suggest that our method can be used in future studies to disentangle 2 H‐fractionation processes, for improving mechanistic δ 2 Hne models for leaf and tree‐ring cellulose and for further development of δ 2 Hne in plant carbohydrates as a potential proxy for climate, hydrology, plant metabolism and physiology. Abstract : Here, we present a hot water‐vapor equilibration method that is suitable for high‐throughput δ 2 Hne analysis of sugar and starch. Additionally, we show first insights into differences of δ 2 Hne values within and between C3, C4 and CAM plant species growing under the same constant climatic conditions.