Amyloid-like Prep1 peptides exhibit reversible blue-green-red fluorescence in vitro and in living cells. Issue 30 (17th March 2021)

• Record Type: Journal Article
• Authors: Monti, Alessandra; Bruckmann, Chiara; Blasi, Francesco; Ruvo, Menotti; Vitagliano, Luigi; Doti, Nunzianna
• Is Part Of: Chemical communications. Volume 57:Issue 30(2021)
• Page Start: 3720
• Page End: 3723
• Publication Date: 2021-03-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc01145f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Florescence emission of the amyloid-like aggregates of the PREP1-based peptide in different contexts. The column of letters is the sequence of the peptide. Abstract : PREP1-based peptides form amyloid-like aggregates endowed with an intrinsic blue-green-red fluorescence with an unusual sharp maximum at 520 nm upon excitation with visible light under physiological conditions. The peptide PREP1[117-132], whose sequence does not contain aromatic residues, presents a pH-dependent and reversible fluorescence, in line with its structural transition from β-sheet rich aggregates to α-helix structures. These findings further demonstrate that the non-canonical fluorescence exhibited by amyloids is an articulated phenomenon.