A reactive peptide interface for site-selective cysteine bioconjugation. Issue 26 (1st March 2021)

• Record Type: Journal Article
• Authors: Tuang, Suan; Dieppa-Matos, Diomedes; Zhang, Chi; Shugrue, Christopher R.; Dai, Peng; Loas, Andrei; Pentelute, Bradley L.
• Is Part Of: Chemical communications. Volume 57:Issue 26(2021)
• Page Start: 3227
• Page End: 3230
• Publication Date: 2021-03-01
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc00095k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A nine-residue cysteine-based reactive peptide interface enables rapid site-selective modification of proteins in water. Abstract : We report aqueous, site-selective modification of proteins using a reactive peptide interface comprising a nine-residue sequence. This interface is the fastest (second-order rate constant of 152 M −1 s −1 ) catalyst-free, cysteine-based method for modifying proteins available to date, and enables near-quantitative labeling of antibodies in cell lysate.