Brevibacillus humidisoli sp. nov., a moderately thermoalkaliphilic and halotolerant species isolated from riverside soil. Issue 3 (24th March 2023)

• Record Type: Journal Article
• Authors: Lee, Jae Ha; Shin, You Me; Ra, Jun Sik; Kim, Seung Bum
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 73:Issue 3(2023)
• Publication Date: 2023-03-24
• Subjects: Brevibacillus humidisoli -- halotolerant -- moderate thermoalkaliphilic -- riverside soil; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005802
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, aerobic, rod-shaped, motile, and endospore-forming bacterial strain designated MMS20-4M-10-Y T was isolated from riverside soil and subjected to taxonomic characterization. Strain MMS20-4M-10-Y T was moderately thermophilic, alkaliphilic and halotolerant, as the strain grew at 25–50 °C (optimum, 45 °C), at pH 7.0–10.0 (optimum, pH 8.0) and in the presence of 0–6 % NaCl (optimum, 0 %). Analysis of 16S rRNA gene sequences indicated that MMS20-4M-10-Y T fell into a phylogenetic cluster belonging to the genus Brevibacillus . Strain MMS20-4M-10-Y T showed the highest 16S rRNA gene sequence similarity to Brevibacillus marinus SCSIO 07484 T (96.7 %). Based on the reults of orthologous average nucleotide identity analysis, MMS20-4M-10-Y T was again mostly related to B. marinus SCSIO 07484 T with 78.0 % identity, which also shared the highest average nucleotide identity of 68.0 %. In contrast, the digital DNA–DNA relatedness analysis indicated that Aneureibacillus migulanus DSM 2895 T was the closest species with 29.5 % similarity. The genome-based analyses indicated that all compared species showed low genomic relatedness with MMS20-4M-10-Y T . The major fatty acids of the strain were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0, the major respiratory quinone was MK-7, the diagnostic polar lipids were phosphatidyl- N -methylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and diagnostic cell-wall diamino acid was meso -diaminopimelic acid, which was consistent with the general chemotaxonomic features of the genus. The total length of the genome was 4.91 Mbp and the DNA G+C content was 51.8 mol%. Based on both phenotypic and phylogenetic evidence, strain MMS20-4M-10-Y T should be classified as representing a novel species of the genus Brevibacillus, for which a name Brevibacillus humidisoli sp. nov. (type strain=MMS20-4M-10-Y T =KCTC 43333 T =LMG 32359 T ) is proposed.