PYL8 ABA receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by ABA. (16th October 2020)

• Record Type: Journal Article
• Authors: Garcia-Maquilon, Irene; Coego, Alberto; Lozano-Juste, Jorge; Messerer, Maxim; de Ollas, Carlos; Julian, Jose; Ruiz-Partida, Rafael; Pizzio, Gaston; Belda-Palazón, Borja; Gomez-Cadenas, Aurelio; Mayer, Klaus F X; Geiger, Dietmar; Alquraishi, Saleh A; Alrefaei, Abdulwahed F; Ache, Peter; Hedrich, Rainer; Rodriguez, Pedro L
• Editors: Jones, Matt
• Is Part Of: Journal of experimental botany. Volume 72:Number 2(2021)
• Page Start: 757
• Page End: 774
• Publication Date: 2020-10-16
• Subjects: ABA receptor -- abiotic stress -- abscisic acid -- AHG1 -- date palm -- Phoenix dactylifera -- PP2C -- PYL8; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/eraa476
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: The identification of those prevalent abscisic acid (ABA) receptors and molecular mechanisms that trigger drought adaptation in crops well adapted to harsh conditions such as date palm ( Phoenix dactylifera, Pd) sheds light on plant–environment interactions. We reveal that PdPYL8-like receptors are predominantly expressed under abiotic stress, with Pd27 being the most expressed receptor in date palm. Therefore, subfamily I PdPYL8-like receptors have been selected for ABA signaling during abiotic stress response in this crop. Biochemical characterization of PdPYL8-like and PdPYL1-like receptors revealed receptor- and ABA-dependent inhibition of PP2Cs, which triggers activation of the pRD29B-LUC reporter in response to ABA. PdPYLs efficiently abolish PP2C-mediated repression of ABA signaling, but loss of the Trp lock in the seed-specific AHG1-like phosphatase PdPP2C79 markedly impairs its inhibition by ABA receptors. Characterization of Arabidopsis transgenic plants that express PdPYLs shows enhanced ABA signaling in seed, root, and guard cells. Specifically, Pd27-overexpressing plants showed lower ABA content and were more efficient than the wild type in lowering transpiration at negative soil water potential, leading to enhanced drought tolerance. Finally, PdPYL8-like receptors accumulate after ABA treatment, which suggests that ABA-induced stabilization of these receptors operates in date palm for efficient boosting of ABA signaling in response to abiotic stress. Abstract : Date palm response to abiotic stress is triggered through PYL8-like ABA receptors that are stabilized by the hormone, which boosts plant adaptation to drought mediated by ABA.