Ocular Delivery of Predatory Bacteria with Cryomicroneedles Against Eye Infection. Issue 21 (8th September 2021)

• Record Type: Journal Article
• Authors: Cui, Mingyue; Zheng, Mengjia; Wiraja, Christian; Chew, Sharon Wan Ting; Mishra, Arti; Mayandi, Venkatesh; Lakshminarayanan, Rajamani; Xu, Chenjie
• Is Part Of: Advanced science. Volume 8:Issue 21(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-08
• Subjects: cryomicroneedles -- drug delivery -- eye infection -- ocular disease -- predatory bacteria; Science -- Periodicals; 505
• DOI: 10.1002/advs.202102327
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus ( B. bacteriovorus ) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long‐term storage studies, and they successfully impede the growth of gram‐negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders. Abstract : This report introduces a cryo‐delivery platform inside which the predation ability of Bdellovibrio bacteriovorus is highly reserved to impede the development of gram‐negative infections.