Gut microbiota profile of patients with acute coronary syndrome, effect of the most frequent comorbidities. (25th November 2020)

• Record Type: Journal Article
• Authors: Martin De Miguel, I; Fernandez, A.I; Lozano, N; Gutierrez-Ibanes, E; Sanz Ruiz, R; Diez-Delhoyo, F; Vazquez, S; Reigadas, E; Garcia De Viedma, D; Elizaga Corrales, J; Fernandez-Aviles, F.J; Bermejo, J
• Is Part Of: European heart journal. Volume 41:(2020)Supplement 2
• Publication Date: 2020-11-25
• Subjects: Acute Coronary Syndromes: Inflammation; Cardiology -- Periodicals; Heart -- Diseases -- Periodicals; 616.12005
• DOI: 10.1093/ehjci/ehaa946.1566
• Languages: English
• ISSNs: 0195-668X

Abstract

Abstract: Introduction: Gut microbiota dysbiosis may modify the inflammatory state, which could trigger the development of acute coronary syndrome (ACS). Moreover, cardiovascular risk factors may alter the microbiota profile involved in ACS development. Purpose: To address the potential role of alterations in microbiota as triggers of ACS and their cardiovascular comorbidities in patients with ACS. Methods: Patients admitted for ACS were prospectively included. Stool samples were collected in the first 24 hours after admission. For microbiota analysis, Operational Taxonomic Unit (OTUs) method from V4 amplicon of 16S rRNA was employed. Identification, quantification, clusterization and taxonomic classification of OTUs was performed in Mothur through RDP database. ACS patient microbiota was compared with the microbiota of 10 healthy controls. The microbiota changes analyses were evaluated with ANOVA analysis. Results: 41 ACS patients with a mean age of 62±11 years old were included. 34 (83%) were males, 17 (42%) suffered arterial hypertension, 18 (44%) dyslipidaemia, 37 (90%) diabetes mellitus and 16 (39%) were current smokers. A multidimensional scaling model allowed us to clearly delineate different clusters between patients with ACS and healthy controls. This was due to important differences in the main phylum of the human microbiota (Table) Bacteroidetes, Firmicutes / Bacteroidetes ratio and Actinobacteria. In addition, there was a significant decrease in protective Cyanobacteria (p=0.03) and Proteobacteria (p=0.04) in men. Hypertensive and dyslipidaemic patients had a significantly higher amount of Firmicutes, Clostridia (p=0.05) and Erysipelotrichia (p=0.03) classes, respectively. Patients with diabetes had significantly less protective Proteobacteria (p=0.002) and Spirochaetes (p=0.04). Finally, smoking was associated with a significant increase in pathogenic Lentisphaerae (p=0.04). Conclusions: Patients with ACS have clear pathogenic alterations of their gut microbiota. Classical cardiovascular risk factors induce different patterns of gut dysbiosis. Funding Acknowledgement: Type of funding source: Public Institution(s). Main funding source(s): Carlos III Health Institute