Rice recruits Sphingomonas strain HJY-rfp via root exudate regulation to increase chlorpyrifos tolerance and boost residual catabolism. (14th May 2021)

• Record Type: Journal Article
• Authors: Feng, Fayun; Zhan, Honglin; Wan, Qun; wang, Ya; Li, Yong; Ge, Jing; Sun, Xing; Zhu, Hong; Yu, Xiangyang
• Editors: Gifford, Miriam
• Is Part Of: Journal of experimental botany. Volume 72:Number 15(2021)
• Page Start: 5673
• Page End: 5686
• Publication Date: 2021-05-14
• Subjects: Agrochemical stress -- catabolism -- chlorpyrifos -- oxidative damage -- plant-endophyte interaction -- rice -- root exudation; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erab210
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : Agrochemical stress causes rice to actively establish pesticide-specific plant–endophyte interactions to detoxify contaminants and boost pesticide catabolism for better survival under polluted conditions via root exudate regulation. Abstract: Inoculation with pollution-degrading endophytes boosts the catabolism of residual contaminants and promotes the pollution adaptation of host plants. We investigated the interaction pattern between Sphingomonas strain HJY- rfp, a chlorpyrifos-degrading endophytic bacterium, and rice ( Oryza sativa ) under pesticide stress using hydroponic cultivation. We observed a notable trend of endophytic root colonization in rice plants treated with 10 mg l −1 chlorpyrifos solution, and after 24 h the migration of HJY- rfp enhanced the chlorpyrifos degradation rate in leaves and stems by 53.36% and 40.81%, respectively. Critically, the rice root exudate profile (organic acids and amino acids) changed under chlorpyrifos stress, and variations in the contents of several components affected the chemotactic behaviour of HJY- rfp . HJY- rfp colonization dramatically activated defensive enzymes, which enabled efficient scavenging of reactive oxygen species, and led to 9.8%, 22.5%, and 41.9% increases in shoot length, fresh weight, and accumulation of total chlorophyll, respectively, in rice suffering from oxidative damage by chlorpyrifos. Endophytic colonization caused up-regulation of detoxification genes that have shown a significant positive correlation with chlorpyrifos degradation in vivo . Collectively, our results demonstrate that agrochemical stress causes plants to actively recruit specific symbiotic microbes to detoxify contaminants and survive better under pollution conditions.