Ultra-marathon training induces vasculoprotector microRNA-125a-5p expression. (25th November 2020)

• Record Type: Journal Article
• Authors: Postula, M; Eyileten, C; Soplinska, A; Zareba, L; Jarosz-Popek, J; Fitas, Z; Czajka, P; Malek, L
• Is Part Of: European heart journal. Volume 41:(2020)Supplement 2
• Publication Date: 2020-11-25
• Subjects: Prevention: Exercise; Cardiology -- Periodicals; Heart -- Diseases -- Periodicals; 616.12005
• DOI: 10.1093/ehjci/ehaa946.2876
• Languages: English
• ISSNs: 0195-668X

Abstract

Abstract: Introduction: Cardiovascular diseases are the leading cause of death worldwide. According to the World Health Organization data, they are responsible for approximately 31% of deaths annually. It is estimated that nearly 80% of premature CVDs are preventable by modification of lifestyle including regular physical activity. Endurance training can be described as long-time activity characterized by high dynamic and low to high power and was proven beneficial in CVD prevention. Several miRNAs, including miR-125a-5p had been reported to be regulated in response to exercise in healthy humans. Additionally, Let-7 family members are involved in initiation and development of inflammatory response and up-regulated after training. Aim of study: Our aim was to determine the differences in inflammatory-related miRNA expression between ultramarathon runners and healthy controls. Materials and methods: Three circulation inflammation-related miRNA related were chosen based on literature search. RNA was extracted from plasma samples using the mirVANA PARIS Kit and quality of extracted material was assessed using a fluorometric assay. RT-PCR was performed using the Taqman advance protocol on a high throughput thermal cycler. Mann Whitney U test was used for unpaired comparison and Spearman's Correlation was used for the database analysis with miRNA expressions, p<0.05. Results: Let-7e, miR-125a-5p, and miR-126 were measured in 40 patients (31 ultra-marathon runners vs 9 control volunteers) We found that control volunteers had significantly lower miR-125a-5p expression levels than ultra-marathon runners (p=0.03). Additionally, miR-125a-5p showed strong positive correlation with number of ultra-marathons run in life, and number of runs exceeding 100km (r=0.473, p=0.01 and r=0.403, p=0.03, respectively). Besides, Let-7e was positively correlated with VE max (maximum ventilation) (r=0.449, p=0.01). Conclusions: Our study showed that endurance training can increase the miR-125a-5p expression and this result is in the line of previous observation since miR-125a-5p reported as vasculoprotector and were elevated in response to all-out high-intensity running. Funding Acknowledgement: Type of funding source: Public grant(s) – National budget only. Main funding source(s): "Preludium" from the National Science Center, Poland