Cell Behavior Changes and Enzymatic Biodegradation of Hybrid Electrospun Poly(3‐hydroxybutyrate)‐Based Scaffolds with an Enhanced Piezoresponse after the Addition of Reduced Graphene Oxide. Issue 8 (1st January 2023)

• Record Type: Journal Article
• Authors: Chernozem, Roman V.; Pariy, Igor; Surmeneva, Maria A.; Shvartsman, Vladimir V.; Planckaert, Guillaume; Verduijn, Joost; Ghysels, Stef; Abalymov, Anatolii; Parakhonskiy, Bogdan V.; Gracey, Eric; Gonçalves, Amanda; Mathur, Sanjay; Ronsse, Frederik; Depla, Diederik; Lupascu, Doru C.; Elewaut, Dirk; Surmenev, Roman A.; Skirtach, Andre G.
• Is Part Of: Advanced healthcare materials. Volume 12:Issue 8(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-01-01
• Subjects: biodegradation -- piezoelectric -- poly(3‐hydroxybutyrate) -- reduced graphene oxide -- tissue engineering; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202201726
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: This is the first comprehensive study of the impact of biodegradation on the structure, surface potential, mechanical and piezoelectric properties of poly(3‐hydroxybutyrate) (PHB) scaffolds supplemented with reduced graphene oxide (rGO) as well as cell behavior under static and dynamic mechanical conditions. There is no effect of the rGO addition up to 1.0 wt% on the rate of enzymatic biodegradation of PHB scaffolds for 30 d. The biodegradation of scaffolds leads to the depolymerization of the amorphous phase, resulting in an increase in the degree of crystallinity. Because of more regular dipole order in the crystalline phase, surface potential of all fibers increases after the biodegradation, with a maximum (361 ± 5 mV) after the addition of 1 wt% rGO into PHB as compared to pristine PHB fibers. By contrast, PHB‐0.7rGO fibers manifest the strongest effective vertical (0.59 ± 0.03 pm V −1 ) and lateral (1.06 ± 0.02 pm V −1 ) piezoresponse owing to a greater presence of electroactive β ‐phase. In vitro assays involving primary human fibroblasts reveal equal biocompatibility and faster cell proliferation on PHB‐0.7rGO scaffolds compared to pure PHB and nonpiezoelectric polycaprolactone scaffolds. Thus, the developed biodegradable PHB‐rGO scaffolds with enhanced piezoresponse are promising for tissue‐engineering applications. Abstract : This work reveals the advantages of biodegradable piezoelectric polymer‐based materials for tissue‐engineering applications. The addition of reduced graphene oxide nanoflakes into electrospun poly(3‐hydrobutyrate) fibrous scaffolds allows a significant increase in both surface potential and piezoresponse, without any alteration of their biodegradation rate. The enhanced piezoresponse provides the improved cell proliferation on the surface of hybrid scaffolds.