Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions. Issue 9 (3rd February 2023)

• Record Type: Journal Article
• Authors: Koo, Kyeong‐Mo; Kim, Chang‐Dae; Kim, Huijung; Cho, Yeon‐Woo; Suhito, Intan Rosalina; Kim, Tae‐Hyung
• Is Part Of: Advanced science. Volume 10:Issue 9(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-02-03
• Subjects: drug screening -- electrochemical detection -- live cell sensing -- metabolic reaction -- stem cell senescence; Science -- Periodicals; 505
• DOI: 10.1002/advs.202207084
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Direct detection of cellular redox signals has shown immense potential as a novel living cell analysis tool. However, the origin of such signals remains unknown, which hinders the widespread use of electrochemical methods for cellular research. In this study, the authors found that intracellular metabolic pathways that generate adenosine triphosphate (ATP) are the main contributors to extracellularly detectable electrochemical signals. This is achieved through the detection of living cells (4, 706 cells/chip, linearity: 0.985) at a linear range of 7, 466–48, 866. Based on this discovery, the authors demonstrated that the cellular signals detected by differential pulse voltammetry (DPV) can be rapidly amplified with a developed medium containing metabolic activator cocktails (MACs). The DPV approach combined with MAC treatment shows a remarkable performance to detect the effects of the anticancer drug CPI‐613 on cervical cancer both at a low drug concentration (2 µm ) and an extremely short treatment time (1 hour). Furthermore, the senescence of mesenchymal stem cells could also be sensitively quantified using the DPV+MAC method even at a low passage number (P6). Collectively, their findings unveiled the origin of redox signals in living cells, which has important implications for the characterization of various cellular functions and behaviors using electrochemical approaches. Abstract : This study provides novel insights into the origin of extracellularly detectable electrochemical signals of living cells. The proposed differential pulse voltammetry + metabolic activator cocktail (DPV+MAC) method, which activates key metabolic reactions in living cells, is effectively applied for rapid drug screening and non‐destructive detection of stem cell senescence.