AtPFA-DSP5 interacts with MPK3/MPK6 and negatively regulates plant salt responses. (2nd December 2021)

• Record Type: Journal Article
• Authors: Xin, Jing; Guo, Shanshan; Zhang, Xiaolei; Tian, Jiahui; Sun, Yu; Shang, Jian-Xiu
• Is Part Of: Plant signaling & behavior. Volume 16:Number 12(2021)
• Publication Date: 2021-12-02
• Subjects: Arabidopsis -- salt stress -- protein tyrosine phosphatase -- AtPFA-DSP5; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2021.2000808
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: Protein tyrosine phosphatases play essential roles in plant growth and development and in plant responses to biotic or abiotic stresses. We recently demonstrated that an atypical dual-specificity protein tyrosine phosphatase in plants, AtPFA-DSP3 (DSP3), negatively regulates plant salt tolerance. Here, we report that a homolog of DSP3, AtPFA-DSP5 (DSP5), affects the response of plants to high-salt conditions. A loss-of-function mutant of DSP5 showed reduced sensitivity to salt treatment at the seed germination and vegetative stages of development while a gain-of-function mutant of DSP5 showed increased sensitivity to salt stress. The salt responses of dsp3dsp5 double-mutant plants were similar to those of dsp3 and dsp5 single-mutant plants. Gel overlay and firefly luciferase complementation assays showed that DSP5 interacts with MPK3 and MPK6 in vitro and in vivo . These results indicate that DSP5 is a novel negative regulator of salt responses in Arabidopsis that interacts directly with MPK3 and MPK6.