Occurrence of Staphylococcus spp. in the wastewaters from Iran: Diversity, antimicrobial resistance, and virulence potential. (3rd February 2023)

• Record Type: Journal Article
• Authors: Ranjbar Omid, Mahsa; Jamali, Houshang; Kafilzadeh, Farshid; Borjian, Amir; Arzanlou, Mohsen
• Is Part Of: Journal of water and health. Volume 21:Number 2(2023)
• Page Start: 178
• Page End: 191
• Publication Date: 2023-02-03
• Subjects: coagulase-negative Staphylococci -- coagulase-positive Staphylococci -- drug resistance -- pathogenic factors -- sewage -- slaughterhouse; Water quality management -- Periodicals; Water -- Pollution -- Environmental aspects -- Periodicals; Environmental health -- Periodicals; Water quality -- Health aspects -- Periodicals; Water -- Health aspects -- Periodicals; Water -- Pollution -- Health aspects -- Periodicals; Public Health; Water Pollution -- prevention & control; Quality Control; Water Microbiology; Water Supply -- standards; Health & Medicine (General); Hydrology; Environmental health; Water -- Health aspects; Water -- Pollution -- Environmental aspects; Water -- Pollution -- Health aspects; Water quality -- Health aspects; Water quality management; Water; Gezondheid; Periodical; Periodicals; 363.61
• DOI: 10.2166/wh.2023.199
• Languages: English
• ISSNs: 1477-8920

Abstract

Abstract: The prevalence, antibiotic resistance, and virulence characteristics of Staphylococci from hospitals, livestock, municipals, and poultry wastewaters were investigated in Ardabil, Iran. From 155 staphylococcal isolates, 44.5% were coagulase-positive Staphylococcus (CoPS) and 55.5% were coagulase-negative Staphylococcus (CoNS) spp. Both CoPS and CoNS species were mainly found in hospital and poultry wastewater samples. The most prominent CoPS and CoNS species were Staphylococcus aureus at 80% and Staphylococcus xylosus at 37%. Methicillin resistance was found in 2% of S. aureus isolates. Overall, 49.2% of CoPS and 47.6% of CoNS isolates exhibited multidrug resistance phenotypes. CoPS isolates were the most resistant to penicillin (89%) and erythromycin (62%) and CoNS isolates exhibited the highest resistance to erythromycin (55%) and tetracycline (49%). Inducible clindamycin resistance was detected in 11% of S. aureus isolates. The ermC and aac genes were detected as the most common macrolide–lincosamide–streptogramin B and aminoglycoside-resistance encoding genes in 82.5 and 22.5% of S. aureus isolates, respectively. Most of the S. aureus isolates were positive for multiple virulence factors. The methicillin-resistant S. aureus isolates belonged to SCC mec type V. A new spa type t19215 was also identified. The occurrence of multidrug-resistant S. aureus with diverse genetic resistance and virulence background in wastewater is of great health concern. HIGHLIGHTS: Ten coagulase-negative and four coagulase-positive Staphylococcus spp. were identified in wastewaters in Iran. 50% of both coagulase-negative and coagulase-positive Staphylococcus spp. isolates were multidrug-resistant. Occurrence of methicillin-resistant S. aureus was rare in wastewater samples in Iran. S. aureus isolates with high virulence potentials were common. A new spa type t19215 was identified in S. aureus isolates. Graphical Abstract