TNF-α regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Mezawa, Masaru; Tsuruya, Yuto; Yamaguchi, Arisa; Yamazaki-Takai, Mizuho; Kono, Tetsuro; Okada, Hiroyuki; McCulloch, Christopher A.; Ogata, Yorimasa
• Is Part Of: Cell adhesion & migration. Volume 16:Issue 1(2022)
• Page Start: 13
• Page End: 24
• Publication Date: 2022-12-31
• Subjects: TNF-α -- gingival epithelial -- cell adhesion -- cell migration -- laminin 5 -- basal lamina -- extracellular matrix; Cell adhesion -- Periodicals; Cell migration -- Periodicals; Cell interaction -- Periodicals; 574.876
• DOI: 10.1080/19336918.2022.2029237
• Languages: English
• ISSNs: 1933-6918

Abstract

ABSTRACT: Laminin 5, type 4 collagen, and α6β4 integrin contribute to the formation of hemidesmosomes in the epithelia of periodontal tissues, which is critical for the development and maintenance of the dentogingival junction. As it is not known whether TNF-α alters the composition of the epithelial pericellular matrix, human gingival epithelial cells were cultured in the presence or absence of TNF-α. Treatment with TNF-α accelerated epithelial cell migration and closure of in vitro wounds. These data indicate unexpectedly, that TNF-α promotes the formation of the pericellular matrix around epithelial cells and enhances adhesion of epithelial cells to the underlying matrix, properties which are important for cell migration and the integrity of the dentogingival junction.