Can Kidney Organoid Xenografts Accelerate Therapeutic Development for Genetic Kidney Disorders?. Issue 2 (7th February 2023)

• Record Type: Journal Article
• Authors: Kuo, Ting-Chun; Cabrera-Barragan, Dalia N.; Lopez-Marfil, Marta; Lopez-Cantu, Diana O.; Lemos, Dario R.
• Is Part Of: Journal of the American Society of Nephrology. Volume 34:Issue 2(2023)
• Page Start: 184
• Page End: 190
• Publication Date: 2023-02-07
• Subjects: genetic kidney disease -- stem cell -- organoids
• DOI: 10.1681/ASN.2022080862
• Languages: English
• ISSNs: 1046-6673

Abstract

Abstract : A number of genetic kidney diseases can now be replicated experimentally, using kidney organoids generated from human pluripotent stem cells. This methodology holds great potential for drug discovery. Under in vitro conditions, however, kidney organoids remain developmentally immature, develop scarce vasculature, and may contain undesired off-target cell types. Those critical deficiencies limit their potential as disease-modeling tools. Orthotopic transplantation under the kidney capsule improves the anatomic maturity and vascularization of kidney organoids, while reducing off-target cell content. The improvements can translate into more accurate representations of disease phenotypes and mechanisms in vivo . Recent studies using kidney organoid xenografts highlighted the unique potential of this novel methodology for elucidating molecular mechanisms driving monogenic kidney disorders and for the development ofnovel pharmacotherapies.