Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1. Issue 1 (4th December 2019)

• Record Type: Journal Article
• Authors: Zhu, Yanfeng; Chen, Weihui; Guan, Weiqun; Fang, Yihong; Hao, Chunfeng
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 47:Issue 1(2019)
• Page Start: 1932
• Page End: 1937
• Publication Date: 2019-12-04
• Subjects: Arsenic trioxide -- Id-1 -- tongue squamous cell carcinoma -- proliferation -- apoptosis; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2019.1613419
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: Objective: Our study aims to investigate the effect of arsenic trioxide (As2 O3 ) on proliferation and apoptosis of Tca8113 tongue squamous carcinoma cells. Methods: Cell proliferation and the expression of Id-1 mRNA in Tca8113 cells after treatment with different concentrations of As2 O3 were detected by MTT and qRT-PCR, respectively. The expression of Id-1, cell proliferation and apoptosis in Id-1 silencing Tca8113 cells were detected by qRT-PCR, Western blot, MTT and flow cytometry, respectively. The pcDNA 3.1-Id-1 overexpression vector was transfected into Tca8113 cells combination with 3 μmol/L As2 O3 . The detection of cell proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in transfected Tca8113 cells were performed by MTT, flow cytometry and Western blot assay, respectively. Results: As2 O3 of different concentration could inhibit the proliferation of Tca8113 cells and IC50 value was 3.004 ± 0.2379 μmol/L. The expression of Id-1 mRNA was down-regulated in Tca8113 cells treated with 3 μmol/L As2 O3 for 48 h. The results of qRT-PCR, Western blot, MTT and flow cytometry indicated that the expression level of Id-1 and cell proliferation ability were decreased while the apoptosis rate was increased in Tca8113 cells after transfection of Id-1 siRNA. Overexpression of Id-1 could attenuate the inhibition or promotion of As2 O3 on proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in Tca8113 cells. Conclusion: As2 O3 could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1.