Three regions of the NIP5;1 promoter are required for expression in different cell types in Arabidopsis thaliana root. (2nd December 2021)

• Record Type: Journal Article
• Authors: Tanaka, Mayuki; Fujiwara, Toru
• Is Part Of: Plant signaling & behavior. Volume 16:Number 12(2021)
• Publication Date: 2021-12-02
• Subjects: Promoter -- boric acid -- transporter -- tissue-specific expression -- root; Plant ecophysiology -- Periodicals; Plant cellular signal transduction -- Periodicals; Plant cellular signal transduction; Plant ecophysiology; Periodicals; Electronic journals; 581
• DOI: 10.1080/15592324.2021.1993654
• Languages: English
• ISSNs: 1559-2316

Abstract

ABSTRACT: Arabidopsis thaliana NIP5;1, a boric acid diffusion facilitator, is involved in the acquisition of boron (B) from soil for growth under B limitation. AtNIP5;1 is expressed mainly in roots, where its expression is highest in the root cap and elongation zone. Here, we studied the role of the AtNIP5;1 promoter in the expression of this gene in roots. We fused a series of AtNIP5;1 promoter variants with deleted 5′-fragments to the GUS reporter gene and investigated the expression patterns by histochemical staining. We found that three regions of the AtNIP5;1 promoter are required for specific expression in the root cap and elongation zone (−880 to −863 bp from the translation start site), distal side of the differentiation zone (−747 to −722 bp), and basal side of the differentiation zone (−661 and −621 bp). The results suggest that at least three regions of the AtNIP5;1 promoter each confer different cell-type-specific expression.