Angiopellosis as an Alternative Mechanism of Cell Extravasation. (21st July 2016)

• Record Type: Journal Article
• Authors: Allen, Tyler A.; Gracieux, David; Talib, Maliha; Tokarz, Debra A.; Hensley, M. Taylor; Cores, Jhon; Vandergriff, Adam; Tang, Junnan; de Andrade, James B.M.; Dinh, Phuong-Uyen; Yoder, Jeffrey A.; Cheng, Ke
• Is Part Of: Stem cells. Volume 35:Number 1(2017:Jan.)
• Page Start: 170
• Page End: 180
• Publication Date: 2016-07-21
• Subjects: Extravasation -- Angiopellosis -- Transmigration -- Stem cell infusion -- Diapedesis; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.2451
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract : Stem cells possess the ability to home in and travel to damaged tissue when injected intravenously. For the cells to exert their therapeutic effect, they must cross the blood vessel wall and enter the surrounding tissues. The mechanism of extravasation injected stem cells employ for exit has yet to be characterized. Using intravital microscopy and a transgenic zebrafish line Tg(fli1a:egpf) with GFP-expressing vasculature, we documented the detailed extravasation processes in vivo for injected stem cells in comparison to white blood cells (WBCs). While WBCs left the blood vessels by the standard diapedesis process, injected cardiac and mesenchymal stem cells underwent a distinct method of extravasation that was markedly different from diapedesis. Here, the vascular wall undergoes an extensive remodeling to allow the cell to exit the lumen, while the injected cell remains distinctively passive in activity. We termed this process Angio-pello-sis, which represents an alternative mechanism of cell extravasation to the prevailing theory of diapedesis. Video Highlight: https://youtu.be/i5EI-ZvhBps