Efficacy and Initial Safety Profile of CXCL12 Treatment in a Rodent Model of Urinary Sphincter Deficiency. (17th July 2017)

• Record Type: Journal Article
• Authors: Koudy Williams, J.; Dean, Ashley; Lankford, Shannon; Andersson, Karl-Erik
• Is Part Of: Stem cells translational medicine. Volume 6:Number 8(2017)
• Page Start: 1740
• Page End: 1746
• Publication Date: 2017-07-17
• Subjects: Intrinsic urinary sphincter deficiency -- Skeletal muscle precursor cells -- Stress urinary incontinence -- Stromal derived factor-1α -- CXCL12; Stem cells -- Periodicals; Regenerative medicine -- Periodicals; Periodicals; 616.0277405
• DOI: 10.1002/sctm.16-0497
• Languages: English
• ISSNs: 2157-6564

Abstract

Abstract: Disappointing results of skeletal muscle precursor cell (skMPC) therapy for women with intrinsic urinary sphincter deficiency (ISD) associated urinary incontinence has increased interest in alternative sphincter regenerative approaches. This study was to measure the safety and efficacy of the cell homing chemokine CXCL12 versus skMPCs in a rat model of ISD. Thirty-six adult female Sprague Dawley rats were divided into 6 treatment (Tx) conditions: (a) no ISD/noTx [Control]; (b) ISD/noTx; (c) ISD + skMPCs; (d) ISD + 3.5 mg CXCL12; (e) ISD + 7mg CXCL12; and (f) ISD + 14 mg CXCL12. Tx's were injected directly into the sphincter complex 30 days post ISD and rats euthanized 30 days post Tx. Blood samples for measurements of kidney and liver function, white and red blood cell counts, were taken at baseline and at euthanasia. Leak point pressures (LPP) were measured prior to, and sphincter collagen/muscle content measured after, euthanasia. There were no effects of treatments on white or red/white blood cell counts, kidney/liver function tests or histopathology of the urinary sphincter complex or surrounding tissues. ISD lowered LPP 35% and sphincter muscle content by 17% versus control rats. CXCL12, but not skMPC injections, restored both LPP to control values in a dose-dependent fashion. Both skMPCs and CXCL12 restored sphincter muscle content to control values. This chemokine approach may represent a novel therapeutic option for ISD and appears, at least short-term, to produce little clinical or tissue pathology. Abstract : Injected cells can incorporate into the urinary sphincter complex. They can also release growth factors. However, injected CXCL12 will primarily act as a chemokine to mobilize cells from the adjacent tissue and bone marrow to the urinary sphincter complex. This appears to be a more effective and efficient method of stimulating tissue regeneration while bypassing lengthy cell collection and expansion protocols.