Application of a Collagen-Based Membrane and Chondroitin Sulfate-Based Hydrogel Adhesive for the Potential Repair of Severe Ocular Surface Injuries. Issue 6 (1st June 2014)

• Record Type: Journal Article
• Authors: Chae, Jemin J.; Mulreany, Daniel G.; Guo, Qiongyu; Lu, Qiaozhi; Choi, Joseph S.; Strehin, Iossif; Espinoza, Freddy A.; Schein, Oliver; Trexler, Morgana M.; Bower, Kraig S.; Elisseeff, Jennifer H.
• Is Part Of: Military medicine. Volume 179:Issue 6(2014)
• Page Start: 686
• Page End: 694
• Publication Date: 2014-06-01
• Subjects: Surgery, Military -- Societies, etc; Medicine, Military -- Societies, etc; Medicine, Military -- Periodicals; Surgery, Military -- Periodicals; Medicine, Military; Surgery, Military; Military Medicine -- Periodicals; Periodicals; Electronic journals; 616.98023
• DOI: 10.7205/MILMED-D-13-00360
• Languages: English
• ISSNs: 0026-4075

Abstract

ABSTRACT: This study was performed to evaluate the potential of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and collagen-based membrane (collagen vitrigel, CV) combination as a method to treat penetrating ocular injuries on the battlefield and to improve this method with two technologies: an antibiotic releasing CS-PEG adhesive and a corneal shaped CV. Burst testing using porcine cadaveric eyes, high-performance liquid chromatography, the Kirby-Bauer bacterial inhibition test, and CV implantations on the live and cadaveric rabbit eyes were performed. The ocular burst test showed CS-PEG adhesive could successfully repair 5-mm to 6-mm length wounds in the corneal and corneoscleral regions but would require CS-PEG + CV to treat larger wounds similar to those seen on the battlefield. In addition, high performance liquid chromatography and the Kirby-Bauer bacterial inhibition test presented evidence suggesting the vancomycin incorporated CS-PEG could inhibit Staphylococcus infection for 9 days. Furthermore, the curved CV showed an advantage by matching the corneal contour without any wrinkle formation. Although this pilot study showed a limited range of possible applications, we demonstrated that the combination of CS-PEG adhesive + CV is a promising method and the 2 technologies improve their applicability to the special demands of the battlefield.