Resistance and fitness costs in diamondback moths after selection using broflanilide, a novel meta‐diamide insecticide. (16th April 2021)

• Record Type: Journal Article
• Authors: Sun, Xi; Wei, Rui; Li, Linhong; Zhu, Bin; Liang, Pei; Gao, Xiwu
• Is Part Of: Insect science. Volume 29:Number 1(2022)
• Page Start: 188
• Page End: 198
• Publication Date: 2021-04-16
• Subjects: broflanilide -- cross‐resistance -- detoxifying enzymes -- fitness cost -- Plutella xylostella; Insects -- Periodicals; Entomology -- Periodicals; 595.705
• DOI: 10.1111/1744-7917.12917
• Languages: English
• ISSNs: 1672-9609

Abstract

Abstract: The diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an insect pest found around the world that feeds on cruciferous crops. The DBM has become resistant to most insecticides in current use in the field. Broflanilide is a novel meta‐diamide insecticide that binds to a new site on the γ‐aminobutyric acid receptor and very efficiently protects against most pests in the order Lepidoptera, including DBM. In this study, the resistance of a laboratory‐bred susceptible strain of DBM to broflanilide and the fitness costs posed by broflanilide to the DBM were evaluated. The DBM had no obvious resistance to broflanilide after 10 generations of selection. The realized heritability h 2 was 0.033, suggesting a low risk of resistance developing in this strain. The F10 generation had no cross‐resistance to the insecticides abamectin and endosulfan (which target the γ‐aminobutyric acid receptor) and chlorantraniliprole (which targets a non‐γ‐aminobutyric acid receptor). The specific activities of important detoxification enzymes (cytochrome P450 monooxygenase, esterase, and glutathione S ‐transferase) were not obviously altered. However, the larval stage was prolonged and the adult stage was shortened significantly in F11 generation than the F0 generation. The total preoviposition period TPOP significantly prolonged 1.90 d in F11 generation. The fitness value Rf (0.93) was lower for the F11 generation than the F0 generation. The results indicated that long‐term exposure to broflanilide exerts clear fitness costs in the DBM. This information will be useful in identifying reasonable broflanilide application guidelines for managing broflanilide resistance in the DBM. Abstract : After selection using broflanilide for 10 generations, an indoor susceptible strain of diamondback moth (DBM) showed no resistance to broflanilide, no cross‐resistance to abamectin, endosulfan and chlorantraniliprole, and no obvious changes of detoxifying enzyme activities but decreased fitness. All these indicated low risk of broflanilide resistance in this DBM strain.