Preparation of fluorescent bimodal probe coupled with ultra-small superparamagnetic iron oxide particles. Issue 2 (June 2022)

• Record Type: Journal Article
• Authors: Liu, Qiang; Chen, Song; Hao, Liguo; Li, Chunxiang; Tian, Hongda; Gu, Hongqian; Li, Zhongtao; Wang, Lei; Li, Zhongyuan
• Is Part Of: Journal of Radiation Research and Applied Sciences. Volume 15:Issue 2(2022)
• Page Start: 143
• Page End: 148
• Publication Date: 2022-06
• Subjects: Ultra-small superparamagnetic iron oxide particles -- Fluorescent bimodal probe -- Cell imaging -- Thyroid cancer -- Fe3O4; Ionizing radiation -- Periodicals; Nuclear physics -- Periodicals; Radiation; Radiation; Periodicals; 539.7
• DOI: 10.1016/j.jrras.2022.04.009
• Languages: English
• ISSNs: 1687-8507

Abstract

Abstract: To prepare USPIO-PEG-Cy5.5 targeted bimodal probe for noninvasive diagnosis of thyroid cancer. The characterization was verified, and the physical and chemical properties were discussed. Fe3 O4 was used as the carrier. Fluorescent Cy5.5 was linked with polyethylene glycol (PEG). A small amount of Cy5.5-NHS fluorescent powder were dissolved in dimethyl sulfoxide solution. PEG-Fe3 O4 nanoparticles were centrifuged and ultra-filtrated. The nanoparticles were resuspended with phosphate buffer to change the pH. The Cy5.5 fluorescence was added to the Fe3 O4 particles and incubated at 37 °C for 2 h. Characterization test of Cy5.5-PEG-Fe3 O4 and relaxation rate measurement were performed. Fro cells were used to perform cell imaging experiment. CCK-8 assay was carried out to detect the cytotoxicity. The bimodal molecular probe Cy5.5-PEG-Fe3 O4 was synthesized successfully. Transmission electron microscopy showed that the particle size of the probe was 18.8 ± 3.5 nm, the water and particle size of the nanoparticles was 35.7 ± 18.6 nm, and the zeta potential was −18.0 mv. The relaxation rate was 0.044 and the slope was 0.97. The cytotoxicity test showed that the bimodal molecular probe had slight toxicity to fro cells with a concentration-dependent manner. The synthesized Cy5.5-PEG-Fe3 O4 probe has low toxicity, good biocompatibility, stable particle size, which can be used for cell imaging for noninvasive diagnosis of thyroid cancer.