Enhanced Immunosensor Using a Handheld pH Meter for the Point‐of‐Care, Sensitive Detection of Prostate Specific Antigen. Issue 2 (27th September 2021)

• Record Type: Journal Article
• Authors: Gao, Rong; Liu, Bingqian; Luo, Dajuan; Su, Yonghuan; Su, Lixia
• Is Part Of: Electroanalysis. Volume 34:Issue 2(2022)
• Page Start: 275
• Page End: 280
• Publication Date: 2021-09-27
• Subjects: point-of-care -- handheld pH meter -- Polyamide-amine dendrite -- Prostate specific antigen; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202100285
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: An enhanced handheld pH meter based immunosensor was proposed for point‐of‐care, quantitative determination of prostate specific antigen (PSA). Monoclonal antibody‐functionalized magnetic beads (MB‐Ab1 ) were prepared to capture PSA in sample, and subsequently bond to polyclonal antibody‐immobilized gold nanoparticles‐polyamide‐amine dendrite‐lead ion (GO‐PAMAM‐Pb 2+ ) and dispersed in H2 S solution. Based on negative Gibbs free energy of formation, PbS (solubility product constant Ksp =8.0×10 −28 was formed by reaction of Pb 2+ and H2 S. Hence, the pH of solution increased due to the H + appeared, and the resulting pH value was monitored by a handheld pH meter. Abstract :