Identification of elite species of Ophiorrhiza utilizing HPTLC analysis and camptothecin as a phytochemical marker: Assessment of extraction effectiveness and organ selection. (March 2023)

• Record Type: Journal Article
• Authors: Konjengbam, Merinashwari; Kumar, Vijay; Dwivedi, Padmanabh; Gangaprasad, A.; Dey, Abhijit; Pandey, Devendra Kumar
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 48(2023)
• Publication Date: 2023-03
• Subjects: Camptothecin -- HPTLC -- Anticancer -- Ultrasonic extraction; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2023.102632
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: Objective: The main objective of this experiment is to find the camptothecin (CPT) content in different parts of three Ophiorrhiza spp. Methods: In this study, three species of Ophiorrhiza viz., O.mungos L., O. hirsutula Wight ex Hook. f. and O.rugosa var prostrata (D.Don) Deb & Mondal were taken. Different parts of plant sample such as inflorescence, leaf, stem, and roots were extracted in methanol (100mg/10 ml w/v) using microwave and screened for camptothecin content by High-performance thin layer chromatography (HPTLC). After the selection of elite species different extraction techniques such as Maceration, Hot water bath extraction, Microwave assisted extraction (MAE), and Ultrasonic assisted extraction (UAE) were performed on the elite species for optimization of extraction technique. Results: High performance thin layer chromatography confirmed the presence of camptothecin in all three species. Highest was observed in O.mungos (0.37%) followed by O.hirsutula (0.11%) and a negligible amount in O.rugosa var prostrata (0.001%). Ultrasonic assisted extraction (UAE) method showed the best results in O.mungos leaves. Graphical abstract: Image 1 Highlights: This study highlights the phytochemical analysis of different plant parts of three Ophiorrhiza spp. Camptothecin was detected as a marker compound through HPTLC. Out of the three Ophiorrhiza spp. analyzed, O.mungos emerged as the most elite species. Leaves of O.mungos was found to have the highest content of camptothecin. Ultrasonic extraction developed as the most efficient extraction technique.