Digital Spatial Profiling of Glomerular Gene Expression in Pauci-Immune Focal Necrotizing Glomerulonephritis. Issue 1 (29th January 2023)

• Record Type: Journal Article
• Authors: Oszwald, André; Mejía-Pedroza, Raúl A.; Schachner, Helga; Aigner, Christof; Rees, Andrew; Kain, Renate
• Is Part Of: Kidney360. Volume 4:Issue 1(2023)
• Page Start: 83
• Page End: 91
• Publication Date: 2023-01-29
• Subjects: Glomerular and tubulointerstitial diseases -- ANCA -- basic science -- gene expression -- glomerular disease -- glomerulonephritis -- glomerulus -- histopathology -- kidney biopsy -- renal pathology -- transcriptional profiling -- vasculitis; 616.61
• DOI: 10.34067/KID.000461202
• Languages: English
• ISSNs: 2641-7650

Abstract

Abstract : Key Points: Pauci-immune focal necrotizing glomerulonephritis (piFNGN) entails heterogeneous glomerular lesions in different stages of evolution. Spatial profiling of glomeruli in piFNGN identifies protein and mRNA signatures that correlate with morphologically distinct lesions. Profiling of individual glomeruli provides insights into the pathogenesis of piFNGN and may identify therapeutic targets or biomarkers. Pauci-immune focal necrotizing glomerulonephritis (piFNGN) involves asynchronous onset and progression of injurious lesions in biopsies. Pathologists can describe this heterogeneity within a biopsy, but translating the information into prognostic or expression analyses is challenging. Understanding the underlying molecular processes could improve treatment; however, bulk or single-cell transcriptomic analyses of dissociated tissue disregard the heterogeneity of glomerular injury. We characterize protein and mRNA expression of individual glomeruli in 20 biopsies from 18 patients with antineutrophil cytoplasmic antibody-associated piFNGN using the NanoString digital spatial profiling (DSP) platform. For this purpose, circular annotations of glomeruli were analyzed using protein, immuno-oncology RNA, and Cancer Transcriptome Atlas panels ( n =120, 72, and 48 glomeruli, respectively). Histologic evaluation of glomerular patterns of injury was performed in adjacent serial sections. Expression data were processed by log2 transformation, quantile normalization, and batch adjustment. DSP revealed distinct but overlapping gene expression profiles relating to the morphological evolution of injurious lesions, including dynamic expression of various immune checkpoint regulators. Enrichment analysis indicated deregulated pathways that underline known and highlight novel potential mechanisms of disease. Moreover, by capturing individual glomeruli, DSP describes heterogeneity between and within biopsies. We demonstrate the benefit of spatial profiling for characterization of heterogeneous glomerular injury, indicating novel molecular correlates of glomerular injury in piFNGN.