Multiscale microscopy to decipher plant cell structure and dynamics. Issue 6 (29th December 2022)

• Record Type: Journal Article
• Authors: Cui, Yaning; Zhang, Xi; Li, Xiaojuan; Lin, Jinxing
• Is Part Of: New phytologist. Volume 237:Issue 6(2023)
• Page Start: 1980
• Page End: 1997
• Publication Date: 2022-12-29
• Subjects: expansion microscopy -- fluorescence correlation spectroscopy -- light sheet microscopy -- light‐field microscopy -- spectrum and polarization optical tomography -- stimulated Raman scattering microscopy -- structural illumination microscopy -- total internal reflection fluorescence microscopy; Botany -- Periodicals; 580
• DOI: 10.1111/nph.18641
• Languages: English
• ISSNs: 0028-646X

Abstract

Summary: New imaging methodologies with high contrast and molecular specificity allow researchers to analyze dynamic processes in plant cells at multiple scales, from single protein and RNA molecules to organelles and cells, to whole organs and tissues. These techniques produce informative images and quantitative data on molecular dynamics to address questions that cannot be answered by conventional biochemical assays. Here, we review selected microscopy techniques, focusing on their basic principles and applications in plant science, discussing the pros and cons of each technique, and introducing methods for quantitative analysis. This review thus provides guidance for plant scientists in selecting the most appropriate techniques to decipher structures and dynamic processes at different levels, from protein dynamics to morphogenesis.