Cannabidiol protects livers against nonalcoholic steatohepatitis induced by high‐fat high cholesterol diet via regulating NF‐κB and NLRP3 inflammasome pathway. Issue 11 (29th April 2019)

• Record Type: Journal Article
• Authors: Huang, Yuanling; Wan, Ting; Pang, Nengzhi; Zhou, Yujia; Jiang, Xuye; Li, Bangyan; Gu, Yingying; Huang, Yufeng; Ye, Xiaodie; Lian, Hui; Zhang, Zhenfeng; Yang, Lili
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 11(2019:Nov.)
• Page Start: 21224
• Page End: 21234
• Publication Date: 2019-04-29
• Subjects: cannabidiol -- NF‐κB -- NLRP3 inflammasome -- nonalcoholic steatohepatitis; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.28728
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Cannabidiol (CBD), an abundant nonpsychoactive constituent of marijuana, has been reported previously to protect against hepatic steatosis. In this study, we studied further the functions and mechanisms of CBD on liver inflammation induced by HFC diet. Mice feeding an HFC diet for 8 weeks were applied to test the protective effect of CBD on livers. RAW264.7 cells were incubated with LPS + ATP ± CBD to study the mechanisms of the effect of CBD against inflammasome activation. We found that CBD alleviated liver inflammation induced by HFC diet. CBD significantly inhibited the nuclear factor‐κappa B (NF‐κB) p65 nuclear translocation and the activation of nucleotide‐binding domain like receptor protein 3 (NLRP3) inflammasome both in vivo and in vitro studies, which lead to the reduction of the expression of inflammation‐related factors in our studies. In addition, Inhibitor of activation of NF‐κB partly suppressed the NLRP3 inflammasome activation, while adding CBD further inhibited NF‐κB activation and correspondingly suppressed the NLRP3 inflammasome activation in macrophages. In conclusion, the suppression of the activation of NLRP3 inflammasome through deactivation of NF‐κB in macrophages by CBD might be one mechanism of its anti‐inflammatory function in the liver. Abstract : Cannabidiol protects livers against nonalcoholic steatohepatitis induced by HFC diet via regulating nuclear factor‐κappa B (NF‐κB) and nucleotide‐binding domain like receptor protein 3 (NLRP3) inflammasome pathway. Targeting NF‐κB and NLRP3 inflammasome activation in macrophages might be a novel treatment for nonalcoholic steatohepatitis.