Ronin governs the metabolic capacity of the embryonic lineage for post‐implantation development. (13th September 2021)

• Record Type: Journal Article
• Authors: Salewskij, Kirill; Gross‐Thebing, Theresa; Ing‐Simmons, Elizabeth; Duethorn, Binyamin; Rieger, Bettina; Fan, Rui; Chen, Rui; Govindasamy, Niraimathi; Brinkmann, Heike; Kremer, Ludmila; Kuempel‐Rink, Nannette; Mildner, Karina; Zeuschner, Dagmar; Stehling, Martin; Dejosez, Marion; Zwaka, Thomas P; Schöler, Hans R; Busch, Karin B; Vaquerizas, Juan M; Bedzhov, Ivan
• Is Part Of: EMBO reports. Volume 22:Number 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-13
• Subjects: embryo -- metabolism -- implantation -- Ronin -- Thap11; Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.15252/embr.202153048
• Languages: English
• ISSNs: 1469-221X

Abstract

Abstract: During implantation, the murine embryo transitions from a "quiet" into an active metabolic/proliferative state, which kick‐starts the growth and morphogenesis of the post‐implantation conceptus. Such transition is also required for embryonic stem cells to be established from mouse blastocysts, but the factors regulating this process are poorly understood. Here, we show that Ronin plays a critical role in the process by enabling active energy production, and the loss of Ronin results in the establishment of a reversible quiescent state in which naïve pluripotency is promoted. In addition, Ronin fine‐tunes the expression of genes that encode ribosomal proteins and is required for proper tissue‐scale organisation of the pluripotent lineage during the transition from blastocyst to egg cylinder stage. Thus, Ronin function is essential for governing the metabolic capacity so that it can support the pluripotent lineage's high‐energy demands for cell proliferation and morphogenesis. Synopsis: Ronin enables active energy production, which is required for the transition from metabolically "quiet" (pre‐implantation) or "quiescent" (diapause) states into a proliferative (post‐implantation) state of embryo development. The metabolic state of the early embryo switches from a "quiet" into an active (proliferative) phase during implantation. Such transition is also required for the establishment of ES cells from mouse blastocysts. Ronin function is essential for proper mitochondrial cristae formation and function, as well as for fine‐tuning the expression of genes that encode ribosomal proteins. In vitro, loss of Ronin results in the establishment of a reversible quiescent state in ES cells, where naïve pluripotency is promoted. In vivo, Ronin governs the metabolic capacity of the embryo for the rapid growth and morphogenesis following implantation. Abstract : Ronin enables active energy production, which is required for the transition from metabolically "quiet" (pre‐implantation) or "quiescent" (diapause) states into a proliferative (post‐implantation) state of embryo development.