Decellularized breast matrix as bioactive microenvironment for in vitro three‐dimensional cancer culture. Issue 4 (1st November 2018)

• Record Type: Journal Article
• Authors: Jin, Qin; Liu, Gang; Li, Shubin; Yuan, Haihan; Yun, Zhizhong; Zhang, Wenqi; Zhang, Shu; Dai, Yanfeng; Ma, Yuzhen
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 4(2019:Apr.)
• Page Start: 3425
• Page End: 3435
• Publication Date: 2018-11-01
• Subjects: breast cancer (BC) -- decellularized scaffold -- extracellular matrix (ECM) -- three‐dimensional (3D) in vitro culture, tumor microenvironment; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.26782
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Breast cancer (BC) is a leading cause of cancer‐related death in women with unsatisfactory survival rates. Advances in the understanding of the genetic basis of BC provide the opportunity to develop gene‐based medicines capable of treating metastatic diseases. Here, we first demonstrated efficient tissue engineering approaches applied to normal breast and BC extracellular matrix (ECM) starting from decellularized human biopsies to generate a three‐dimensional (3D) bioactive model with the sodium lauryl ether sulfate solution. The decellularized tissues maximized the genetic component removal from tissues and minimally injured ECM structures and native compositions by histology and ECM compositions analyses. Importantly, we proved that the 3D ECM retained tissues biological properties. We demonstrated that after 30 days of recellularization with MCF‐7 cell (human breast adenocarcinoma cell line), the 3D cancer ECM induced an overexpression of epithelial–mesenchymal transition (EMT) and cancer proliferation. Meanwhile, normal ECM from the breast inhibited EMT and cell growth with the inducement of apoptosis. Given the biological activity preserved in the ECM after decellularization, we believe these approaches are powerful tools for future preclinical research for BC and breast development. Abstract : The decellularized tissues of normal human breast tissues and cancer tissues maximized the genetic component removal from tissues and minimally injured extracellular matrix (ECM) structures and native compositions. After 30 days of recellularization with MCF‐7 cell, the three‐dimensional cancer ECM induced an overexpression of epithelial–mesenchymal transition (EMT) and cancer proliferation. Meanwhile, normal ECM from breast inhibited EMT and cell growth with the inducement of apoptosis.