Characterization of an in vitro model to study the possible role of polyomavirus BK in prostate cancer. Issue 7 (4th December 2018)

• Record Type: Journal Article
• Authors: Villani, Sonia; Gagliano, Nicoletta; Procacci, Patrizia; Sartori, Patrizia; Comar, Manola; Provenzano, Maurizio; Favi, Evaldo; Ferraresso, Mariano; Ferrante, Pasquale; Delbue, Serena
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 7(2019:Jul.)
• Page Start: 11912
• Page End: 11922
• Publication Date: 2018-12-04
• Subjects: cytokine production -- in vitro model -- polyomavirus -- prostate Cancer (PCa); Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27871
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Prostate cancer (PCa) is the most common male neoplasms in the Western world. Various risk factors may lead to carcinogenesis, including infectious agents such as polyomavirus BK (BKPyV), which infects the human renourinary tract, establishes latency, and encodes oncoproteins. Previous studies suggested that BKPyV plays a role in PCa pathogenesis. However, the unspecific tropism of BKPyV and the lack of in vitro models of BKPyV‐infected prostate cells cast doubt on this hypothesis. The aim of the present study was to determine whether BKPyV could (a) infect normal and/or tumoral epithelial prostate cells and (b) affect their phenotype. Normal epithelial prostate RWPE‐1 cells and PCa PC‐3 cells were infected with BKPyV for 21 days. Cell proliferation, cytokine production, adhesion, invasion ability, and epithelial‐to‐mesenchymal transition (EMT) markers were analyzed. Our results show that (a) RWPE‐1 and PC‐3 cells are both infectable with BKPyV, but the outcome of the infection varies, (b) cell proliferation and TNF‐α production were increased in BKPyV‐infected RWPE‐1, but not in PC‐3 cells, (c) adhesion to matrigel and invasion abilities were elevated in BKPyV‐infected RWPE‐1 cells, and (d) loss of E‐cadherin and expression of vimentin occurred in both uninfected and infected RWPE‐1 cells. In conclusion, BKPyV may change some features of the normal prostate cells but is not needed for maintaining the transformed phenotype in the PCa cells The fact that RWPE‐1 cells exhibit some phenotype modifications related to EMT represents a limit of this in vitro model. Abstract : BKPyV is able to infect both normal (RWPE‐1) and tumoral (PC‐3) prostate cells, but the infections have different outcomes. Cell proliferation and TNF‐α production were increased in BKPyV‐infected RWPE‐1, but not in PC‐3 cells. Adhesion to matrigel and invasion abilities were elevated in BKPyV‐infected RWPE‐1 cells. Loss of E‐cadherin and expression of vimentin occurred in both uninfected and infected RWPE‐1 cells. In conclusion, BKPyV may change some features of the normal prostate cells but is not needed for maintaining the transformed phenotype in the PCa cells.