Immobilization of BMP‐2, BMP‐7 and alendronic acid on titanium surfaces: Adhesion, proliferation and differentiation of bone marrow‐derived stem cells. Issue 2 (18th October 2019)

• Record Type: Journal Article
• Authors: Kämmerer, Peer W.; Pabst, Andreas M.; Dau, Michael; Staedt, Henning; Al‐Nawas, Bilal; Heller, Martin
• Is Part Of: Journal of biomedical materials research. Volume 108:Issue 2(2020)
• Page Start: 212
• Page End: 220
• Publication Date: 2019-10-18
• Subjects: alendronate -- bio‐functionalization -- BMP‐2 -- BMP‐7 -- BMSC -- cell adhesion -- dental implant -- proliferation -- TiO2 -- titanium; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.36805
• Languages: English
• ISSNs: 1549-3296

Abstract

Abstract: This study analyzed the influence of titanium (TiO2 ) surface modifications with two osteogenic proteins (BMP‐2, BMP‐7) and an anti‐osteoclastic drug (alendronic acid [AA]) on sandblasted/acid‐etched (SLA) and plain TiO2 (PT) on cell adhesion, proliferation and differentiation (alkaline phosphatase [AP] and osteocalcin [OC]) of bone‐marrow derived stem cells (BMSCs) after 1, 3 and 7 days in‐vitro. Initially, AA surfaces showed the highest cell number and surface coverage. At day 3 and 7, BMP and AA‐modified surfaces exhibited a significantly enhanced cell growth. For proliferation, at days 3 and 7, an enhancement on BMP‐2, BMP‐7 and AA‐surfaces was seen. At day 7, SLA also showed a higher proliferation when compared to PT. Initially, AP expression was elevated on SLA and AA surfaces. At days 3 and 7, a significant increased AP expression was seen for SLA, BMP‐2, BMP‐7 and AA discs. For OC, SLA and AA surfaces had the highest expression after 1 day whereas after 3 and 7 days a significant difference was recorded for SLA, BMP‐2, BMP‐7 and AA. In conclusion, a beneficial biological effect of a chemical immobilization method of BMP‐2, BMP‐7 and alendronate onto titanium surfaces on BMSCs was proven.