Specific neural phase relation of serotonin and dopamine modulate the testicular activity in Japanese quail. Issue 3 (2nd August 2018)

• Record Type: Journal Article
• Authors: Banerjee, Somanshu; Chaturvedi, Chandra Mohini
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 3(2019:Mar.)
• Page Start: 2866
• Page End: 2879
• Publication Date: 2018-08-02
• Subjects: connexin‐43 and 14‐3‐3 -- Japanese quail -- specific neural oscillations of serotonin and dopamine -- steroidogenesis -- testis; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27104
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : Specific phase relation of serotonin and dopamine modulate the hypothalamo–hypophyseal–gonadal axis as well as photosexual responses in Japanese quail, but the effect of these specific phase relations on testicular activity and steroidogenesis is not yet been investigated. We hypothesized that temporal phase relation induced alteration in local testicular gonadotropin‐releasing hormone (GnRH)–Gonadotropin‐inhibitory hormone (GnIH) and their receptor system may modulate the testicular activity and steroidogenesis through local (paracrine and autocrine) action. To validate this hypothesis, we have checked the alterations in the expression of gonadotropin‐releasing hormone receptor (GnRH‐R), gonadotropin‐inhibitory hormone receptor (GnIH‐R) messenger RNA (mRNA), growth hormone receptor (GH‐R), proliferating cell nuclear antigen (PCNA), cell communication and gap junctional proteins (14‐3‐3 and connexin‐43 [Cnx‐43]), steroidogenic factor‐1 (SF‐1), steroidogenic acute regulatory (StAR) protein, steroidogenic enzyme (3β‐hydroxysteroid dehydrogenase [3β‐HSD]) in testis as well as androgen receptor (AR) in testis and epididymis of control, 8‐, and 12‐hr quail. Experimental findings clearly indicate the increased expression of GnIH‐R mRNA and suppression of GnRH‐R, GH‐R, PCNA, 14‐3‐3, Cnx‐43, SF‐1, StAR, 3β‐HSD in testis as well as AR in testis and epididymis in 8‐hr quail, while 12‐hr quail exhibited the opposite results that is significantly decreased expression of GnIH‐R mRNA and increased expression of GnRH‐R, GH‐R, PCNA, 14‐3‐3, Cnx‐43, SF‐1, StAR, 3β‐HSD in testis as well as AR in testis and epididymis. The significantly increased intratesticular testosterone has been observed in the 12‐hr quail while, 8‐hr quail showed opposite result. Hence, it can be concluded that 12‐hr quail showed significantly increased testicular activity and steroidogenesis while opposite pattern was observed in 8‐hr quail. Abstract : 1. 8‐ and 12‐hr specific phase relations of 5‐HTP and L‐DOPA differentially modulate testicular responses, that is testicular activity or steroidogenesis and result in testicular atrophy or regression and hypertrophy/stimulation respectively. 2. Increased expression of GnRH‐R, GH‐R, PCNA, Cnx‐43, 14‐3‐3, SF‐1, StAR, 3β‐HSD and AR in testis along with the decreased GnIH‐R, may lead to testicular hypertrophy/increased steroidogenesis in sexually active and full breeding 12‐hr quail. 3. While, opposite expression pattern of these factors suppresses the testicular activity/steroidogenesis and results in testicular atrophy in sexually quiescent, nonbreeding 8‐hr quail.