PURB is a positive regulator of amino acid‐induced milk synthesis in bovine mammary epithelial cells. Issue 5 (26th October 2018)

• Record Type: Journal Article
• Authors: Huo, Nan; Yu, Mengmeng; Li, Xueying; Zhou, Chenjian; Jin, Xin; Gao, Xuejun
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 5(2019:May)
• Page Start: 6992
• Page End: 7003
• Publication Date: 2018-10-26
• Subjects: amino acid -- mammary epithelial cell (MEC) -- mTOR -- purine‐rich element binding protein B (PURB) -- SREBP‐1c; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27452
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Previous studies have implicated that purine‐rich element binding protein B (PURB) is a key regulator of gene transcription and cell physiology. Whether PURB plays a regulatory role in milk synthesis in bovine mammary epithelial cells (BMECs) is not known. We observed that Met and Leu increased PURB expression and nuclear localization. Overexpression of PURB led to increased milk protein and fat synthesis as well as mTOR and SREBP‐1c expression whereas PURB knockdown had the opposite effects. The PI3K inhibitor wortmannin totally abolished the stimulation of Met and Leu on PURB expression, and we further confirmed that PURB was required for Met and Leu to stimulate mTOR phosphorylation and SREBP‐1c expression. We also demonstrated that PURB binds to the promoters of mTOR and SREBP‐1c, and these bindings were increased by Met and Leu stimulation. In summary, our data reveal that PURB is required for amino acids to stimulate mTOR and SREBP‐1c gene expression, and PURB is a positive regulator of amino acid‐induced PI3K‐regulated milk protein and fat synthesis in BMECs. Abstract : Amino acids (Met and Leu) trigger PURB expression in the nucleus via the PI3K signaling and binding to the promoters of mTOR and SREBP‐1c, and PURB is required for amino acids to stimulate the gene expression of mTOR and SREBP‐1c leading to milk protein and fat synthesis in bovine mammary epithelial cells.