SARS-CoV-2 vaccination in rituximab-treated patients: B cells promote humoral immune responses in the presence of T-cell-mediated immunity. Issue 10 (20th July 2021)

• Record Type: Journal Article
• Authors: Mrak, Daniel; Tobudic, Selma; Koblischke, Maximilian; Graninger, Marianne; Radner, Helga; Sieghart, Daniela; Hofer, Philipp; Perkmann, Thomas; Haslacher, Helmuth; Thalhammer, Renate; Winkler, Stefan; Blüml, Stephan; Stiasny, Karin; Aberle, Judith H; Smolen, Josef S; Heinz, Leonhard X; Aletaha, Daniel; Bonelli, Michael
• Is Part Of: Annals of the rheumatic diseases. Volume 80:Issue 10(2021)
• Page Start: 1345
• Page End: 1350
• Publication Date: 2021-07-20
• Subjects: rituximab -- vaccination -- COVID-19; Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2021-220781
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Objectives: Evidence suggests that B cell-depleting therapy with rituximab (RTX) affects humoral immune response after vaccination. It remains unclear whether RTX-treated patients can develop a humoral and T-cell-mediated immune response against SARS-CoV-2 after immunisation. Methods: Patients under RTX treatment (n=74) were vaccinated twice with either mRNA-1273 or BNT162b2. Antibodies were quantified using the Elecsys Anti-SARS-CoV-2 S immunoassay against the receptor-binding domain (RBD) of the spike protein and neutralisation tests. SARS-CoV-2-specific T-cell responses were quantified by IFN-γ enzyme-linked immunosorbent spot assays. Prepandemic healthy individuals (n=5), as well as healthy individuals (n=10) vaccinated with BNT162b2, served as controls. Results: All healthy controls developed antibodies against the SARS-CoV-2 RBD of the spike protein, but only 39% of the patients under RTX treatment seroconverted. Antibodies against SARS-CoV-2 RBD significantly correlated with neutralising antibodies (τ=0.74, p<0.001). Patients without detectable CD19 + peripheral B cells (n=36) did not develop specific antibodies, except for one patient. Circulating B cells correlated with the levels of antibodies (τ=0.4, p<0.001). However, even patients with a low number of B cells (<1%) mounted detectable SARS-CoV-2-specific antibody responses. SARS-CoV-2-specific T cells were detected in 58% of the patients, independent of a humoral immune response. Conclusions: The data suggest that vaccination can induce SARS-CoV-2-specific antibodies in RTX-treated patients, once peripheral B cells at least partially repopulate. Moreover, SARS-CoV-2-specific T cells that evolved in more than half of the vaccinated patients may exert protective effects independent of humoral immune responses.