Cell sorters see things more clearly now. Issue 3 (13th February 2023)

• Record Type: Journal Article
• Authors: Schraivogel, Daniel; Steinmetz, Lars M
• Is Part Of: Molecular systems biology. Volume 19:Issue 3(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2023-02-13
• Subjects: Molecular biology -- Periodicals; Systems biology -- Periodicals; 572.8
• DOI: 10.15252/msb.202211254
• Languages: English
• ISSNs: 1744-4292

Abstract

Abstract : Microscopy and fluorescence‐activated cell sorting (FACS) are two of the most important tools for single‐cell phenotyping in basic and biomedical research. Microscopy provides high‐resolution snapshots of cell morphology and the inner workings of cells, while FACS isolates thousands of cells per second using simple parameters, such as the intensity of fluorescent protein labels. Recent technologies are now combining both methods to enable the fast isolation of cells with microscopic phenotypes of interest, thereby bridging a long‐standing gap in the life sciences. In this Commentary, we discuss the technical advancements made by image‐enabled cell sorting and highlight novel experimental strategies in functional genomics and single‐cell research. Abstract : This Commentary discusses the technical advancements made by image‐enabled cell sorting and highlights novel experimental strategies in functional genomics and single‐cell research.